FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008144643

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008144643
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1742
Total Bases252.4 kbp
Sequences flagged as poor quality0
Sequence length140-145
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGCTTACACATCCCTGAGAACTTTTGCTTGGACTGTAGAACCATGT47227.09529276693456No Hit
GCTGCGCTTACACATCTCTGAGAACTTTTGCTTGGACTGTAGAACCATGT34819.97703788748565No Hit
GCTGCGCTTACACATCTCTTCCACTTCTTTTACTCTGGCATATTTGAGAC30017.221584385763492No Hit
GCTGCGCTTACACATCTCTGCTAAGCTGTGCAGGCTCGATAAGCTGCTTG331.894374282433984No Hit
GCTGCGCTTACACATCTCTGCTAAGCTGCTTGGGGATGACCCGAAGCAGT291.6647531572904706No Hit
GCTGCGCTTACACATCTCTTCTAAGCAGTGGTATCAACGCAGAGTACATG281.6073478760045925Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT241.377726750861079No Hit
GCTGCGCTTACACATCTCTGCTAAGCAGTGGTATCAACGCAGAGTACATG241.377726750861079Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGTTGATTCAAGTGCTTTGGACTGTAGAACCATGTCGTCAGTGTAGATCT201.1481056257175661No Hit
TGCTGATCATTGTTGCTTTGGACTGTAGAACCATGTCGTCAGTGTAGATC191.0907003444316876No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC140.8036739380022963No Hit
GCTGCGCTTACAATCTCTTCCACTTCTTTTACTCTGGCATATTTGAGACT110.6314580941446613No Hit
GCTGCGCTTACACATCTGACGCTGCCGACGACCCATGTACTCTGCGTTGA90.5166475315729048No Hit
GCTGCGCTTACACATCTCTGCTAAGCTGCTTGGGGATGACCCCCATGTAC80.4592422502870264No Hit
GCTGCGCTTACACATCTCTGACATCCCTGAGAACTTTTGCTTGGACTGTA80.4592422502870264No Hit
GTGCGCTTACACATCTCTTCCACTTCTTTTACTCTGGCATATTTGAGACT70.4018369690011481No Hit
GCTGCGCTTACACATCTCTGCTAAGCTGCTTGGGGATGACCCCATGTACT70.4018369690011481No Hit
GCTGCGCTTACACATCTCTGCTAAGCTGCTTGGGGATGACCAAGCAGTGG70.4018369690011481No Hit
GCTGCGCTTACACATCTATCCCCTCATCAACACGCCATCCCCTCATCAAC70.4018369690011481No Hit
GCTGCGCTTACACATCTCTTCTAAGCTGTGCAGGCTCGATAAGCTGCTTG70.4018369690011481No Hit
GCTGCGCTTACACATCCCTAGAACTTTTGCTTGGACTGTAGAACCATGTC70.4018369690011481No Hit
GCTGCGCTTACACATCTCTGAGCCCACGAGACCCCATGTACTCTGCGTTG60.3444316877152698No Hit
GCTGCGCTTACACATCTCTGAGAACTTTTCTTGGACTGTAGAACCATGTC60.3444316877152698No Hit
CAACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGGAAGC60.3444316877152698Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCTGCGCTTACACATCTCTGAGAACTTTGCTTGGACTGTAGAACCATGTC60.3444316877152698No Hit
CAACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGAAGCA50.28702640642939153Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCTGCGTTACACATCTCTTCCACTTCTTTTACTCTGGCATATTTGAGACT50.28702640642939153No Hit
GCTGCGCTTACACATCTCTAACATCCCTGAGAACTTTTGCTTGGACTGTA40.2296211251435132No Hit
GTTGCGCTTACACATCTCTTCCACTTCTTTTACTCTGGCATATTTGAGAC40.2296211251435132No Hit
GCTGCGCTTACACATCTCTGCTAAGCTGTGCAGGCTCGATAAGCAGTGGT30.1722158438576349No Hit
GCTGCGTTTACACATCTCTTCCACTTCTTTTACTCTGGCATATTTGAGAC30.1722158438576349No Hit
GCTGCGCTTACAATCTCTGAGAACTTTTGCTTGGACTGTAGAACCATGTC30.1722158438576349No Hit
GCTGCGCTTACACATCTCTTCCCAAGCAGTGGTATCAACGCAGAGTACAT30.1722158438576349Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGCGCTTACACATCTCTGAGAACTTTTGCTTGGACTGTAGAACCATGTC30.1722158438576349No Hit
GCTGCGCTTACACATCCCTGAAACTTTTGCTTGGACTGTAGAACCATGTC30.1722158438576349No Hit
TCCTATCAGTGGCCGCAAGGCCTAAACAGGAGTTTTGGACTGTAGAACCA30.1722158438576349No Hit
GCTGCGTTTACACATCCCTGAGAACTTTTGCTTGGACTGTAGAACCATGT30.1722158438576349No Hit
GCTGCGCTTACACATCTCGGACATCCCTGAGAACTTTTGCTTGGACTGTA30.1722158438576349No Hit
GCGCGCTTACACATCCCTGAGAACTTTTGCTTGGACTGTAGAACCATGTC20.1148105625717566No Hit
GCTGCGCTTACACATCCTGAGAACTTTTGCTTGGACTGTAGAACCATGTC20.1148105625717566No Hit
GTTGCGCTTACACATCTCTGAGAACTTTTGCTTGGACTGTAGAACCATGT20.1148105625717566No Hit
GCTGCGCTTACACATCCCTGAGAACTTTGCTTGGACTGTAGAACCATGTC20.1148105625717566No Hit
GCTGCGTTTACACATCTCTGAGAACTTTTGCTTGGACTGTAGAACCATGT20.1148105625717566No Hit
GCTGCGCTTACACACCTCTTCTAAGCAGTGGTATCAACGCAGAGTACATG20.1148105625717566Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGCGCTTACACATCCCTGAGAACTTTTGCTTGGACTGTAGAACCATGTC20.1148105625717566No Hit
ACTGCGCTTACACATCCCTGAGAACTTTTGCTTGGACTGTAGAACCATGT20.1148105625717566No Hit
GCTGCGCTTACACATCTCTGCTAAGCTGTGCAGGCTCGATGAGCAGGTCG20.1148105625717566No Hit
GCTGCGCTTACACATCTCCGAGCCCACGAGACCCCATGTACTCTGCGTTG20.1148105625717566No Hit
GCTGCGCTTACACATCTCTCTAAGCAGTGGTATCAACGCAGAGTACATGG20.1148105625717566Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTGCGCTTACACATCTCTTCCAAGCAGTGGTATCAACGCAGAGTACATG20.1148105625717566Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTGCGCTTACACATCTCTGAGACTTTTGCTTGGACTGTAGAACCATGTC20.1148105625717566No Hit
GCTGCGCTTACACATCTCTGCTAAGCTGCTTGGGGATGACCCGGAAGCAG20.1148105625717566No Hit
GCTGGGCTTACACATCTCTGAGAACTTTTGCTTGGACTGTAGAACCATGT20.1148105625717566No Hit
GCTGCGCTTACACATCTCTGCTAAGCAGTGGTATCAACGCAGAGTACATC20.1148105625717566Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTGTGCTTACACATCTCTTCCACTTCTTTTACTCTGGCATATTTGAGAC20.1148105625717566No Hit
GCTGCGCTTACACATCTCTCTAAGCTGCTTGGGGATGACCCGAAGCAGTG20.1148105625717566No Hit
GCTGCGCTTACACATCCCTGAGAACTTTTGCTTGGACTGTAGACCATGTC20.1148105625717566No Hit
GTTGCGCTTACACATCCCTGAGAACTTTTGCTTGGACTGTAGAACCATGT20.1148105625717566No Hit
GCTGCGCTTACACATTCCTGAGAACTTTTGCTTGGACTGTAGAACCATGT20.1148105625717566No Hit
GCTGCGCTTACACATCTCCCCCTCATCAACACATCCCCTCATCAACACGC20.1148105625717566No Hit
GCTGCGCTTACATATCCCTGAGAACTTTTGCTTGGACTGTAGAACCATGT20.1148105625717566No Hit
GCTGCGCTTACACATCCCTGAGAACTTTTCTTGGACTGTAGAACCATGTC20.1148105625717566No Hit
GCTCGCTTACACATCTCTTCCACTTCTTTTACTCTGGCATATTTGAGACT20.1148105625717566No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)