FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008144644

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008144644
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2815
Total Bases407.5 kbp
Sequences flagged as poor quality0
Sequence length135-145
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAAAAGTTCTCAGGGATGTGTAAGCGCAGCGGCTAAAGACCAAGTCTCT59221.03019538188277No Hit
GCAAAAGTTCTCAGAGATGTGTAAGCGCAGCGGCTAAAGACCAAGTCTCT51918.43694493783304No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACAC47116.731793960923625No Hit
CGCCAGGCAGGAATCATTCAGGCCTTGCAGAATTTGGGACTCTGCCATAT1605.683836589698046No Hit
AGCAACAATGATCAGCAGGCTAAAGACCAAGTCTCTGCTACCGTATGCTA1033.6589698046181174No Hit
AGCACTTGAATCAACAGGCTAAAGACCAAGTCTCTGCTACCGTATGCTAC923.268206039076377No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA622.202486678507993No Hit
AACTCCTGTTTAGGCCTTGCAGAATTTGGGACTCTGCCATATTCTTTCCG521.8472468916518652No Hit
GCAAAAGTTCTCAGGGATGTGTAAGCGCAGCCTTCCCATGTACTCTGCGT471.669626998223801No Hit
GCAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGC291.0301953818827707No Hit
GCAAAAGTTCTCAGGGATGTCAGAGATGTGTAAGCGCAGCGGCTAAAGAC270.9591474245115453No Hit
GCAAAAGTTCTCAGGGATGTCGGCAAAAGTTCTCAGGGATGTAGGCAAAA210.7460035523978685No Hit
AGCACTTGAATAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC180.6394316163410302No Hit
GCAAAGTTCTCAGGGATGTGTAAGCGCAGCGGCTAAAGACCAAGTCTCTG170.6039076376554174No Hit
AACTCCTGTTTAGGCCTTGCGGCCACTGATAGGAGGCTAAAGACCAAGTC140.49733570159857904No Hit
GCAAAAGTTCTCAGGGATGTTAGAGATGTGTAAGCGCAGCGGCTAAAGAC130.4618117229129663No Hit
GCAAAAGTTCTCAGGGATGTCCGAGATGTGTAAGCGCAGCGGCTAAAGAC110.39076376554174064No Hit
GCAAAAGTTCTCAGGGATGTTGATGAGGGGATCCCATGTACTCTGCGTTG110.39076376554174064No Hit
GCAAAAGTTCTCAGGGATGTCGATGAGGGGATCCCATGTACTCTGCGTTG110.39076376554174064No Hit
GCAAAAGTTCTCAGGGATGTCGTGTTGATGAGGGGATCCCATGTACTCTG110.39076376554174064No Hit
GCAAAAGTTCTCAGGGATGTGTAAGCGCAGCGGCTAAAGACAAAGTCTCT90.3197158081705151No Hit
GAAAAGTTCTCAGGGATGTGTAAGCGCAGCGGCTAAAGACCAAGTCTCTG80.2841918294849023No Hit
CGCCAGGCAGGAATCATTGGTATCAACGCAGAGTACATGGAAGCAGTGGT80.2841918294849023No Hit
GCAAAGTTCTCAGAGATGTGTAAGCGCAGCGGCTAAAGACCAAGTCTCTG80.2841918294849023No Hit
CGCCAGGCAGGAATCATTGGTATCAACGCAGAGTACATGGGAAGCAGTGG70.24866785079928952No Hit
GCAAAAGTTCTCAGGGATGTCCTCTGCGTTGATACCACTGCTTCCCATGT70.24866785079928952Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCAAAAGTTCTCAGAGATGTGTAAGCGCAGCGGCTAAAGACAAAGTCTCT60.21314387211367672No Hit
GCAAAAGTTCTCAGGGATGTCGTGTTGATGAGGGGATGGCGTGTTGATGA60.21314387211367672No Hit
GCAAAAGTTCTCAGGGATGTCGATGAGGGGATGGCGTGTTGATGAGGGGA60.21314387211367672No Hit
GCAAAAGTTCTCAGGGATGTGTAAGCGCAGCCTCCCCATGTACTCTGCGT50.17761989342806395No Hit
CGCCAGGCAGGAATCATTCACACTGACGACATGGTTCTACAGTCCAAGCA50.17761989342806395No Hit
GCAAAAGTTCTAGGGATGTGTAAGCGCAGCGGCTAAAGACCAAGTCTCTG50.17761989342806395No Hit
GCAAAAGTTCTCAGGGATGTCCCACTGACGACATGGTTCTACAGTCCAAG50.17761989342806395No Hit
GAAAAGTTCTCAGAGATGTGTAAGCGCAGCGGCTAAAGACCAAGTCTCTG50.17761989342806395No Hit
GCAAAAGTTCTCAGGGATGTTGATGAGGGGATGGCGTGTTGATGAGGGGA40.14209591474245115No Hit
GCAAAAGTTCTCAGGGATGTCACACTGACGACATGGTTCTACAGTCCAAG40.14209591474245115No Hit
GCAAAAGTTCTCAGGGGTGTGTAAGCGCAGCGGCTAAAGACCAAGTCTCT40.14209591474245115No Hit
GCCAGGCAGGAATCATTCAGGCCTTGCAGAATTTGGGACTCTGCCATATT40.14209591474245115No Hit
AAGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACA40.14209591474245115No Hit
GCAAAAGTTCTCAGGGATGTGTAAGCGCAGTGGCTAAAGACCAAGTCTCT40.14209591474245115No Hit
AGCACTTGAATCAACAGGCTAAAGACCAAGTCTCTGCTACCGTATGTTAC30.10657193605683836No Hit
GCAAAAGTTCTCAGAGATGTGTAAGCGCAGCGGCTAAAGACCAAGACTCT30.10657193605683836No Hit
GCAAAAGTTCTCAGAGATGTGTAAGCGCAACGGCTAAAGACCAAGTCTCT30.10657193605683836No Hit
GCAAAAGTTCTCAGGGATGTGTAAGCGCAGCCTTAGAAGAGATGTGTAAG30.10657193605683836No Hit
GCAAAAGTTCTCAGGGATGTAGGCAAAAGTTCTCAGGGATGTAGGCAAAA30.10657193605683836No Hit
GCAAAAGTTCTCAGGGATGTCGGCAAAAGTTCTCAGAGATGTGTAAGCGC30.10657193605683836No Hit
AGCAACAATGATCAGCAGGCTAAAGACCAAGTCTCTGCTACCGTATGTTA30.10657193605683836No Hit
GCAAAAGTTCTCAGGGATGTGTAAGCGCAACGGCTAAAGACCAAGTCTCT30.10657193605683836No Hit
GCAAAAGTTCTCAGGATGTGTAAGCGCAGCGGCTAAAGACCAAGTCTCTG30.10657193605683836No Hit
CGCCAGGCAGGAATCATTCAGGCCTTGTAGAATTTGGGACTCTGCCATAT30.10657193605683836No Hit
GCAAAAGTCTCAGGGATGTGTAAGCGCAGCGGCTAAAGACCAAGTCTCTG30.10657193605683836No Hit
GCAAAAGTTCTCAGAGATGTGTAAACGCAGCGGCTAAAGACCAAGTCTCT30.10657193605683836No Hit
GCAAAAGTTCTCAGGGATGTGTAAGCGCACGGCTAAAGACCAAGTCTCTG30.10657193605683836No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG30.10657193605683836Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAAAAGTTCTCAGAGATGTGTAAGCGCACGGCTAAAGACCAAGTCTCTG30.10657193605683836No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)