FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008144645

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008144645
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2815
Total Bases407.9 kbp
Sequences flagged as poor quality0
Sequence length139-145
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGCTTACACATCCCTGAGAACTTTTGCTTGGACTGTAGAACCATGT60821.598579040852574No Hit
GCTGCGCTTACACATCTCTGAGAACTTTTGCTTGGACTGTAGAACCATGT54019.182948490230906No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC46516.518650088809945No Hit
GCTGCGCTTACACATCTCTTCCACTTCTTTTACTCTGGCATATTTGAGAC1465.186500888099467No Hit
TGCTGATCATTGTTGCTTTGGACTGTAGAACCATGTCGTCAGTGTAGATC1073.8010657193605684No Hit
TGTTGATTCAAGTGCTTTGGACTGTAGAACCATGTCGTCAGTGTAGATCT1003.5523978685612785No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT551.9538188277087036No Hit
GCTGCGCTTACACATCTCTGAGAACTTTTGCCTACATCCCTGAGAACTTT270.9591474245115453No Hit
GCTGCGCTTACACATCTCTGACATCCCTGAGAACTTTTGCTTGGACTGTA270.9591474245115453No Hit
CAACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACA240.8525754884547069No Hit
GCTGCGCTTACACATCTCTTCTAAGCAGTGGTATCAACGCAGAGTACATG240.8525754884547069Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTGCGCTTACACATCCCTGAGAACTTTGCTTGGACTGTAGAACCATGTC180.6394316163410302No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAAATCAAGTCAAATTT140.49733570159857904No Hit
TCCTATCAGTGGCCGCAAGGCCTAAACAGGAGTTTTGGACTGTAGAACCA140.49733570159857904No Hit
GCTGCGCTTACACATCTCTGCTAAGCTGTGCAGGCTCGATAAGCTGCTTG130.4618117229129663No Hit
GCTGCGCTTACACATCTCTAACATCCCTGAGAACTTTTGCTTGGACTGTA130.4618117229129663No Hit
GCTGCGCTTACACATCTCGGACATCCCTGAGAACTTTTGCTTGGACTGTA120.42628774422735344No Hit
GCTGCGCTTACACATCTCTTCATCAACACGCCATCCCCTCATCAACACGC110.39076376554174064No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAATCAAGTCAAATTTC100.3552397868561279No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAAAATCAAGTCAAATT90.3197158081705151No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAGAGTCTGGAGTATTTCCTC80.2841918294849023No Hit
GCTGCGCTTACACATCCCTGAGAACTTTTCTTGGACTGTAGAACCATGTC80.2841918294849023No Hit
GCTGCGCTTACACATCTCTGAGAACTTTGCTTGGACTGTAGAACCATGTC80.2841918294849023No Hit
GCTGCGCTTACACATCTCTGCTAAGCAGTGGTATCAACGCAGAGTACATG70.24866785079928952Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAATCAAGTCAAATTTCT70.24866785079928952No Hit
GCTGCGCTTACAATCTCTTCCACTTCTTTTACTCTGGCATATTTGAGACT60.21314387211367672No Hit
GCTGCGCTTACACATCTCTTCCCTCATCAACACGCCATCCCCTCATCAAC50.17761989342806395No Hit
GCTGCGCTTACACATCTCTGAGAACTTTTCTTGGACTGTAGAACCATGTC50.17761989342806395No Hit
GCTGCGCTTACACATCTATCCCCTCATCAACACGCCATCCCCTCATCAAC50.17761989342806395No Hit
GCTGCGCTTACACATCCCTAGAACTTTTGCTTGGACTGTAGAACCATGTC50.17761989342806395No Hit
GCTGCGCTTACACATCTCTGAGAACTTTTGCCGACATCCCTGAGAACTTT50.17761989342806395No Hit
GCTGCGCTTACACATCTCTTCCCAAGCAGTGGTATCAACGCAGAGTACAT40.14209591474245115Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTGCGCTTACACATCTGGTCATCCCCAAGCAGCTGCGTTGATACCACTG40.14209591474245115No Hit
ACTGCGCTTACACATCCCTGAGAACTTTTGCTTGGACTGTAGAACCATGT40.14209591474245115No Hit
GTTGCGCTTACACATCCCTGAGAACTTTTGCTTGGACTGTAGAACCATGT40.14209591474245115No Hit
TGCTGATCATTGTTGTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT40.14209591474245115No Hit
GTGCGCTTACACATCTCTGAGAACTTTTGCTTGGACTGTAGAACCATGTC40.14209591474245115No Hit
GCTGCGCTTACACACCCCTGAGAACTTTTGCTTGGACTGTAGAACCATGT40.14209591474245115No Hit
GCTGCGCTTACACATCTCTGCTAAGCTGTGCAGGCTCGATAAGCAGTGGT30.10657193605683836No Hit
GCTGCGCTTACACATCCTGAGAACTTTTGCTTGGACTGTAGAACCATGTC30.10657193605683836No Hit
GTTGCGCTTACACATCTCTGAGAACTTTTGCTTGGACTGTAGAACCATGT30.10657193605683836No Hit
GCTGCGTTTACACATCTCTGAGAACTTTTGCTTGGACTGTAGAACCATGT30.10657193605683836No Hit
GTTGTTTAGAATGAGGAGAGAGGCCCTGGGCTTCACTTACTCTTCATTCA30.10657193605683836No Hit
GCTGCGCTTACACATCCCTGAGAACTTTTGCTTGGACCGTAGAACCATGT30.10657193605683836No Hit
TGCTGATCATTGTTGCTTTGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT30.10657193605683836No Hit
TGTTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC30.10657193605683836No Hit
GCTGCGCTTACACATCTCTGCTAAGCTGCTTGGGGATGACCCAAGCAGTG30.10657193605683836No Hit
TGTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT30.10657193605683836No Hit
GTGCGCTTACACATCCCTGAGAACTTTTGCTTGGACTGTAGAACCATGTC30.10657193605683836No Hit
GCTGCGCTTACATATCTCTTCCACTTCTTTTACTCTGGCATATTTGAGAC30.10657193605683836No Hit
GCTGCGCTTACACATCTCTTCCAAGCAGTGGTATCAACGCAGAGTACATG30.10657193605683836Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTGCGCTTACACATCTCTGCTAAGCTGCTTGGGGATGACCAAGCAGTGG30.10657193605683836No Hit
GCTGCGCTTACACATCTCTTCTAAGCTGTGCAGGCTCGATAAGCTGCTTG30.10657193605683836No Hit
GCTGCGTTACACATCTCTTCCACTTCTTTTACTCTGGCATATTTGAGACT30.10657193605683836No Hit
TGCTGATCTTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT30.10657193605683836No Hit
GCTGCGCTTACACATCCCTGAGACTTTTGCTTGGACTGTAGAACCATGTC30.10657193605683836No Hit
TGCTGATATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT30.10657193605683836No Hit
TGCTGATCATTGTTGCTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC30.10657193605683836No Hit
GCTGCGCTTACACATCTCTTCTAAGGCTGCGCTTACACATCCCTGAGAAC30.10657193605683836No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)