FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008144650

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008144650
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2500
Total Bases361.8 kbp
Sequences flagged as poor quality0
Sequence length141-145
%GC53

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAAAAGTTCTCAGGGATGTGTAAGCGCAGCGGCTAAAGACCAAGTCTCT51520.599999999999998No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACAC43917.560000000000002No Hit
GCAAAAGTTCTCAGAGATGTGTAAGCGCAGCGGCTAAAGACCAAGTCTCT40516.2No Hit
AGCACTTGAATCAACAGGCTAAAGACCAAGTCTCTGCTACCGTATGCTCG1506.0No Hit
AACTCCTGTTTAGGCCTTGCAGAATTTGGGACTCTGCCATATTCTTTCCG1064.24No Hit
AGCAACAATGATCAGCAGGCTAAAGACCAAGTCTCTGCTACCGTATGCTC1004.0No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA833.32No Hit
CGCCAGGCAGGAATCATTCAGGCCTTGCAGAATTTGGGACTCTGCCATAT562.2399999999999998No Hit
GCAAAAGTTCTCAGGGATGTGTAAGCGCAGCCTTCCCATGTACTCTGCGT562.2399999999999998No Hit
GCAAAAGTTCTCAGGGATGTCAGAGATGTGTAAGCGCAGCGGCTAAAGAC271.08No Hit
GCAAAAGTTCTCAGGGATGTCGGCAAAAGTTCTCAGGGATGTAGGCAAAA220.88No Hit
AACTCCTGTTTAGGCCTTGCGGCCACTGATAGGAGGCTAAAGACCAAGTC170.6799999999999999No Hit
GCAAAAGTTCTCAGGGATGTCGTGTTGATGAGGGGATCCCATGTACTCTG140.5599999999999999No Hit
GCAAAAGTTCTCAGGGATGTCGATGAGGGGATCCCATGTACTCTGCGTTG130.52No Hit
GCAAAAGTTCTCAGGGATGTTAGAGATGTGTAAGCGCAGCGGCTAAAGAC100.4No Hit
GCAAAAGTTCTCAGGGATGTTGATGAGGGGATCCCATGTACTCTGCGTTG100.4No Hit
GCAAAGTTCTCAGAGATGTGTAAGCGCAGCGGCTAAAGACCAAGTCTCTG90.36No Hit
CGCCAGGCAGGAATCATTCACACTGACGACATGGTTCTACAGTCCAAGCA90.36No Hit
GCAAAGTTCTCAGGGATGTGTAAGCGCAGCGGCTAAAGACCAAGTCTCTG70.27999999999999997No Hit
CAAAAGTTCTCAGAGATGTGTAAGCGCAGCGGCTAAAGACCAAGTCTCTG60.24No Hit
GCAAAAGTTCTCAGGGATGTCGTGTTGATGAGGGGATGGCGTGTTGATGA60.24No Hit
GCAAAAGTTCTCAGAGATGTGTAAACGCAGCGGCTAAAGACCAAGTCTCT50.2No Hit
GCAAAAGTTCTCAGGGATGTGTAAGCGCAACGGCTAAAGACCAAGTCTCT50.2No Hit
GCAAAAGTTCTCAGGGATGTGTAAGCGCAGCGGCTAAAGACAAAGTCTCT50.2No Hit
AGCACTTGAATAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC50.2No Hit
GCAAAAGTTCTCAGGGATGTCCTCTGCGTTGATACCACTGCTTCCCATGT40.16Clontech SMARTer II A Oligonucleotide (96% over 25bp)
AACTCCTGTTTAGGCTTTGCGGCCACTGATAGGAGGCTAAAGACCAAGTC40.16No Hit
GCAAAAGTTCTCAGGGATGTCACACTGACGACATGGTTCTACAGTCCAAG40.16No Hit
GCAAAAGTTTCAGGGATGTGTAAGCGCAGCGGCTAAAGACCAAGTCTCTG40.16No Hit
GCAAAAGTTCTCAGGGATGTGTAAGCGCACGGCTAAAGACCAAGTCTCTG40.16No Hit
CGCCAGGCAGGAATCATTGGTATCAACGCAGAGTACATGGAAGCAGTGGT40.16No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACCCACAC40.16No Hit
GCAAAAGTTCTCAGGGATGTGAGCAACTCTGCGTTGATACCACTGCTTCC40.16Clontech Universal Primer Mix Long (96% over 25bp)
GCAAAAGTTTCAGAGATGTGTAAGCGCAGCGGCTAAAGACCAAGTCTCTG40.16No Hit
AGCACTTGAATCAACAGGCTTCGGAGACTGAGCCATTTTCAAACTCACAC40.16No Hit
GCAAAAGTTCTCAGGGATGTGTAAACGCAGCGGCTAAAGACCAAGTCTCT40.16No Hit
CAAAAGTTCTCAGGGATGTGTAAGCGCAGCGGCTAAAGACCAAGTCTCTG40.16No Hit
GCAAAAGTTCTCAGGGATGTCGAGATGTGTAAGCGCAGCGGCTAAAGACC40.16No Hit
GCAAAAGTTCTCAGAGATGTGTAAGCGCAGCGGCTAAAGACAAAGTCTCT30.12No Hit
GCAAAAGTTCTCAGGATGTGTAAGCGCAGCGGCTAAAGACCAAGTCTCTG30.12No Hit
GCAAAAGTTCTCAGGGATGTACTCTGCGTTGATACCACTGCTTCCCATGT30.12Clontech Universal Primer Mix Long (96% over 26bp)
GAAAAGTTCTCAGGGATGTGTAAGCGCAGCGGCTAAAGACCAAGTCTCTG30.12No Hit
GCAAAAGTTCTCAGAGATGTGTAGCGCAGCGGCTAAAGACCAAGTCTCTG30.12No Hit
ACTCCTGTTTAGGCCTTGCAGAATTTGGGACTCTGCCATATTCTTTCCGT30.12No Hit
AGCACTTGATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC30.12No Hit
CGCCAGGCAGGAATCATTGGTATCAACGCAGAGTACATGGGAAGCAGTGG30.12No Hit
GCAGTGGTATCAACGCAGAGTACATGGGGTCTCGTGGGCTCAGAGATGTG30.12Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAAAAGTTCTCAGGGATGTGAGCAACTCTGCGTTGATACCACTGCCCCA30.12Clontech SMART CDS Primer II A (95% over 22bp)
GCAAAAGTTCTCAGGGATGTGTAAGCGCAGCCTTGCAGAGATGTGTAAGC30.12No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)