FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008144657

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008144657
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1536
Total Bases222.6 kbp
Sequences flagged as poor quality0
Sequence length143-145
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGCTTACACATCCCTGAGAACTTTTGCTTGGACTGTAGAACCATGT40526.3671875No Hit
GCTGCGCTTACACATCTCTGAGAACTTTTGCTTGGACTGTAGAACCATGT37724.544270833333336No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC1388.984375No Hit
GCTGCGCTTACACATCTCTTCCACTTCTTTTACTCTGGCATATTTGAGAC634.1015625No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT583.7760416666666665No Hit
TGCTGATCATTGTTGCTTTGGACTGTAGAACCATGTCGTCAGTGTAGATC362.34375No Hit
GCTGCGCTTACACATCTCTGACATCCCTGAGAACTTTTGCTTGGACTGTA291.8880208333333333No Hit
GCTGCGCTTACACATCTCTTCTAAGCAGTGGTATCAACGCAGAGTACATG261.6927083333333333Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGTTGATTCAAGTGCTTTGGACTGTAGAACCATGTCGTCAGTGTAGATCT221.4322916666666665No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAAATCAAGTCAAATTT201.3020833333333335No Hit
CAACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACA171.1067708333333335No Hit
GCTGCGCTTACACATCCCTGAGAACTTTGCTTGGACTGTAGAACCATGTC100.6510416666666667No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAATCAAGTCAAATTTC100.6510416666666667No Hit
TCCTATCAGTGGCCGCAAGGCCTAAACAGGAGTTTTGGACTGTAGAACCA100.6510416666666667No Hit
GCTGCGCTTACACATCTCTGCTAAGCAGTGGTATCAACGCAGAGTACATG70.4557291666666667Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTGCGCTTACACATCTCTAACATCCCTGAGAACTTTTGCTTGGACTGTA70.4557291666666667No Hit
GCTGCGCTTACACATCTCGGACATCCCTGAGAACTTTTGCTTGGACTGTA70.4557291666666667No Hit
GCTGCGCTTACACATCTCTTCATCAACACGCCATCCCCTCATCAACACGC60.390625No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAAAATCAAGTCAAATT50.32552083333333337No Hit
GTGCGCTTACACATCCCTGAGAACTTTTGCTTGGACTGTAGAACCATGTC50.32552083333333337No Hit
GCTGCGCTTACACATCTCTGAGAACTTTGCTTGGACTGTAGAACCATGTC50.32552083333333337No Hit
TGCTGATCATTGTTGCTTTGGGGGTGGGGAAAAGTCTGGAGTATTTCCTC40.26041666666666663No Hit
GCTGCGTTTACACATCCCTGAGAACTTTTGCTTGGACTGTAGAACCATGT40.26041666666666663No Hit
GCTGCGCTTACACATCCCTGAGAACTTTTCTTGGACTGTAGAACCATGTC40.26041666666666663No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAGGTCTGGAGTATTTCCTC30.1953125No Hit
GCTGCGCTTACACATCCCTGAAAACTTTTGCTTGGACTGTAGAACCATGT30.1953125No Hit
GCTGCGTTACACATCTCTGAGAACTTTTGCTTGGACTGTAGAACCATGTC30.1953125No Hit
GCTGCGCTTACACATCCCTGAAACTTTTGCTTGGACTGTAGAACCATGTC30.1953125No Hit
GCTGCGCCTTACACATCTCTGAGAACTTTTGCTTGGACTGTAGAACCATG30.1953125No Hit
TCCTATCAGTGGCCGCAAAGCCTAAACAGGAGTTTTGGACTGTAGAACCA30.1953125No Hit
GCTGCGCTTACACATCTACTCTGACATCCCTGAGAACTTTTGCTTGGACT30.1953125No Hit
GCTGCGCTTACACATCCCTAGAACTTTTGCTTGGACTGTAGAACCATGTC30.1953125No Hit
GCTGCGCTTACACATCTCTGAGAACTTTTGCCGACATCCCTGAGAACTTT30.1953125No Hit
GCTGCGCTTACAATCTCTTCCACTTCTTTTACTCTGGCATATTTGAGACT30.1953125No Hit
GCTGCGCTTACACATCCTGAGAACTTTTGCTTGGACTGTAGAACCATGTC20.13020833333333331No Hit
GTTGCGCTTACACATCTCTGAGAACTTTTGCTTGGACTGTAGAACCATGT20.13020833333333331No Hit
GCTGCGCTTACACATCTCTGAGAACTTTTGTTGGACTGTAGAACCATGTC20.13020833333333331No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAACGTCTGGAGTATTTCCTC20.13020833333333331No Hit
GCTGCGTTTACACATCTCTGAGAACTTTTGCTTGGACTGTAGAACCATGT20.13020833333333331No Hit
GCTGCGCTTACACATCTTTGACATCCCTGAGAACTTTTGCTTGGACTGTA20.13020833333333331No Hit
GCTGCGCTTACACATCTCTGCTAAGCTGCTTGGACTGTAGAACCATGTCG20.13020833333333331No Hit
GCTGCGCTTACACATCTCTGAGAACTTTTGCTTGGACTGTAGAACCATGA20.13020833333333331No Hit
GCTGCGCTTACACATCTCTTCTAAGCTGCTTGGACTGTAGAACCATGTCG20.13020833333333331No Hit
GCTGCGCTTACACATCCCTGAACTTTTGCTTGGACTGTAGAACCATGTCG20.13020833333333331No Hit
GCTGCGCTTACACATCTCGACATCCCTGAGAACTTTTGCTTGGACTGTAG20.13020833333333331No Hit
GCTGCGCTTACACATCTCTGAGAACTTTTGCCTACATCCCTGAGAACTTT20.13020833333333331No Hit
GCTGCGCTTACACATCTCTAGAACTTTTGCTTGGACTGTAGAACCATGTC20.13020833333333331No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAGAAGTCTGGAGTATTTCCTC20.13020833333333331No Hit
GCTGCGCTTACACATCTCTGAGAATTTGCTTGGACTGTAGAACCATGTCG20.13020833333333331No Hit
GCTGCTTACACATCCCTGAGAACTTTTGCTTGGACTGTAGAACCATGTCG20.13020833333333331No Hit
GCTGCGCTTACACATCTCGGACATCCCTAGAACTTTTGCTTGGACTGTAG20.13020833333333331No Hit
GCTGCGCTTACACATCCCTGGGAACTTTTGCTTGGACTGTAGAACCATGT20.13020833333333331No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACCTACTCT20.13020833333333331No Hit
GCTGCGCTTACACATCTCTGAGAACTTTTGCCTGGACTGTAGAACCATGT20.13020833333333331No Hit
GCTGCGCTTACACATCTCTGAAACTTTTGCTTGGACTGTAGAACCATGTC20.13020833333333331No Hit
GTTGCGCTTACACATCTCTTCCACTTCTTTTACTCTGGCATATTTGAGAC20.13020833333333331No Hit
GCTGCGCTTACACATCTCTAACATCCCTGAGAACTTTGCTTGGACTGTAG20.13020833333333331No Hit
GCTGCGCTTACACATCTCTGAGAACGGACATCCCTGAGAACTTTTGCTTG20.13020833333333331No Hit
GCTGCGCTTACACATCTCTTAGAACTTTTGCTTGGACTGTAGAACCATGT20.13020833333333331No Hit
GCTGCGCTTACACATCCCTGAGACTTTTGCTTGGACTGTAGAACCATGTC20.13020833333333331No Hit
TGCTGATCATTTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT20.13020833333333331No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)