FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008144660

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008144660
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2270
Total Bases328.7 kbp
Sequences flagged as poor quality0
Sequence length142-145
%GC54

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAAAAGTTCTCAGGGATGTGTAAGCGCAGCGGCTAAAGACCAAGTCTCT44319.515418502202646No Hit
GCAAAAGTTCTCAGAGATGTGTAAGCGCAGCGGCTAAAGACCAAGTCTCT42818.854625550660792No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACAC2089.162995594713657No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA2089.162995594713657No Hit
CGCCAGGCAGGAATCATTCAGGCCTTGCAGAATTTGGGACTCTGCCATAT934.096916299559472No Hit
AACTCCTGTTTAGGCCTTGCAGAATTTGGGACTCTGCCATATTCTTTCCG903.9647577092511015No Hit
AGCACTTGAATCAACAGGCTAAAGACCAAGTCTCTGCTACCGTACAGCTA763.3480176211453743No Hit
GCAAAAGTTCTCAGGGATGTGTAAGCGCAGCCTTCCCATGTACTCTGCGT763.3480176211453743No Hit
AGCAACAATGATCAGCAGGCTAAAGACCAAGTCTCTGCTACCGTACAGCT522.290748898678414No Hit
GCAAAAGTTCTCAGGGATGTCAGAGATGTGTAAGCGCAGCGGCTAAAGAC441.9383259911894273No Hit
GCAAAAGTTCTCAGGGATGTCGGCAAAAGTTCTCAGGGATGTAGGCAAAA140.6167400881057269No Hit
AACTCCTGTTTAGGCCTTGCGGCCACTGATAGGAGGCTAAAGACCAAGTC130.5726872246696035No Hit
GCAAAAGTTCTCAGGGATGTCGATGAGGGGATCCCATGTACTCTGCGTTG130.5726872246696035No Hit
GCAAAAGTTCTCAGGGATGTTAGAGATGTGTAAGCGCAGCGGCTAAAGAC120.5286343612334802No Hit
GCAAAAGTTCTCAGGGATGTCCGAGATGTGTAAGCGCAGCGGCTAAAGAC100.4405286343612335No Hit
CGCCAGGCAGGAATCATTCACACTGACGACATGGTTCTACAGTCCAAGCA90.39647577092511016No Hit
GCAAAAGTTCTCAGGGATGTCGTGTTGATGAGGGGATGGCGTGTTGATGA80.3524229074889868No Hit
AGCACTTGAATAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC80.3524229074889868No Hit
GCAAAAGTTCTCAGGGATGTCCTCTGCGTTGATACCACTGCTTCCCATGT70.30837004405286345Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCAAAAGTTCTCAGGGATGTCGATGAGGGGATGGCGTGTTGATGAGGGGA60.2643171806167401No Hit
GCAAAAGTTCTCAGAGATGTGTAAGCGCAGCGGCTAAAGACAAAGTCTCT60.2643171806167401No Hit
GCAAAAGTTCTCAGGATGTGTAAGCGCAGCGGCTAAAGACCAAGTCTCTG60.2643171806167401No Hit
GCAAAGTTCTCAGGGATGTGTAAGCGCAGCGGCTAAAGACCAAGTCTCTG60.2643171806167401No Hit
GCAAAAGTTCTCAGGGATGTGTAAGCGCACGGCTAAAGACCAAGTCTCTG50.22026431718061676No Hit
GCAAAAGTTCTCAGGGATGTCGGCAAAAGTTCTCAGAGATGTGTAAGCGC50.22026431718061676No Hit
GCAAAAGTTCTCAGGGATGTTGATGAGGGGATCCCATGTACTCTGCGTTG50.22026431718061676No Hit
AACTCCTGTTTAGGCTTTGCGGCCACTGATAGGAGGCTAAAGACCAAGTC40.1762114537444934No Hit
GGTGTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG40.1762114537444934No Hit
GCAAAGTTCTCAGAGATGTGTAAGCGCAGCGGCTAAAGACCAAGTCTCTG40.1762114537444934No Hit
GCAAAAGTTCTCAGAGATGTGTAAGCGCACGGCTAAAGACCAAGTCTCTG40.1762114537444934No Hit
GCAAAAGTTCTCAGGGATGGCGTGTTGATGAGGGGATGGCGTGTTGATGA40.1762114537444934No Hit
GCAAAAGTTCTCAGGGATGTGTAAGCGCAGCGGCTAAAGACAAAGTCTCT40.1762114537444934No Hit
GCAAAAGTTCTCAGGGATGTTGATGAGGGGATGGCGTGTTGATGAGGGGA30.13215859030837004No Hit
GCAAAAGTTTCAGGGATGTGTAAGCGCAGCGGCTAAAGACCAAGTCTCTG30.13215859030837004No Hit
GCAAAAGTTCTCAGGGATGTCCACTGACGACATGGTTCTACAGTCCAAGC30.13215859030837004No Hit
GCAAAAGTTCTCAGGGATGTGTAAGCGCAGCCTCCCCATGTACTCTGCGT30.13215859030837004No Hit
GCAAAAGTTCTCAGGGATGTCGATGAGGGGATCAAGGAAGCAGTGGTATC30.13215859030837004No Hit
GCAAAAGTTCTCAGGGATGTCGTGTTGATGAGGGGATCAAGGAAGCAGTG30.13215859030837004No Hit
GCAAAAGTTCTCAGGGATGTGTAAGCGCAGCCTTCCCCATGTACTCTGCG30.13215859030837004No Hit
CGCCAGGCAGGAATCATTGGTATCAACGCAGAGTACATGGAAGCAGTGGT30.13215859030837004No Hit
GCAAAAGTTCTCAGAGATGTGTAAGCGCAGCGGCTAAAGACCAACTCTCT30.13215859030837004No Hit
AGCACTTGAATCACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC30.13215859030837004No Hit
GCAAAGTTCTCAGGGATGTCAGAGATGTGTAAGCGCAGCGGCTAAAGACC30.13215859030837004No Hit
GCAAAAGTTCTCAGAGATGTGTAACGCAGCGGCTAAAGACCAAGTCTCTG30.13215859030837004No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCGGGTGGTTCGCAGAAGCAGCA30.13215859030837004No Hit
GCAAAAGTTCTCAGGGATGTCGCGGTGAGTTCAGGCTTTTTCATGGTTTA30.13215859030837004No Hit
GCAAAAGTTCTCAGGGATGTCGTGTTGATGAGGGGATTGCGTGTTGATGA30.13215859030837004No Hit
GCAAAAGTTCTAGGGATGTCAGAGATGTGTAAGCGCAGCGGCTAAAGACC30.13215859030837004No Hit
CGCCAGGCAGGAATCATTACACTGACGACATGGTTCTACAGTCCAAGCAA30.13215859030837004No Hit
GCAAAAGTTCTCAGGGATGTCGAGATGTGTAAGCGCAGCGGCTAAAGACC30.13215859030837004No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)