FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008144661

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008144661
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2270
Total Bases328.9 kbp
Sequences flagged as poor quality0
Sequence length140-145
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGCTTACACATCCCTGAGAACTTTTGCTTGGACTGTAGAACCATGT45620.088105726872246No Hit
GCTGCGCTTACACATCTCTGAGAACTTTTGCTTGGACTGTAGAACCATGT45420.0No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC2149.427312775330396No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT2028.898678414096917No Hit
GCTGCGCTTACACATCTCTTCCACTTCTTTTACTCTGGCATATTTGAGAC883.8766519823788546No Hit
TGTTGATTCAAGTGCTTTGGACTGTAGAACCATGTCGTCAGTGTAGATCT783.4361233480176208No Hit
TGCTGATCATTGTTGCTTTGGACTGTAGAACCATGTCGTCAGTGTAGATC562.4669603524229076No Hit
GCTGCGCTTACACATCTCTGACATCCCTGAGAACTTTTGCTTGGACTGTA441.9383259911894273No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAAATCAAGTCAAATTT281.2334801762114538No Hit
GCTGCGCTTACACATCTCTTCTAAGCAGTGGTATCAACGCAGAGTACATG220.9691629955947136Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTGCGCTTACACATCTCTGCTAAGCTGTGCAGGCTCGATAAGCTGCTTG210.9251101321585903No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAATCAAGTCAAATTTC170.7488986784140969No Hit
GCTGCGCTTACACATCTCTAACATCCCTGAGAACTTTTGCTTGGACTGTA140.6167400881057269No Hit
GCTGCGCTTACACATCTCTGAGAACTTTTGCCTACATCCCTGAGAACTTT130.5726872246696035No Hit
GCTGCGCTTACACATCTCTGCTAAGCTGCTTGGGGATGACCCACGAAGCA130.5726872246696035No Hit
TCCTATCAGTGGCCGCAAGGCCTAAACAGGAGTTTTGGACTGTAGAACCA120.5286343612334802No Hit
GCTGCGCTTACACATCTCTGCTAAGCTGCTTGGGGATGACCCAAGGGGGA110.4845814977973568No Hit
GCTGCGCTTACACATCTCGGACATCCCTGAGAACTTTTGCTTGGACTGTA110.4845814977973568No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAAAATCAAGTCAAATT110.4845814977973568No Hit
GCTGCGCTTACACATCTCTGCTAAGCAGTGGTATCAACGCAGAGTACATG100.4405286343612335Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTGTTTAGAATGAGGAGAGAGGCCCTGGGCTTCACTTACTCTTCATTCA90.39647577092511016No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAATCAAGTCAAATTTCT70.30837004405286345No Hit
GCTGCGCTTACACATCCTGAGAACTTTTGCTTGGACTGTAGAACCATGTC60.2643171806167401No Hit
GCTGCGCTTACACATCCCTGAGAACTTTGCTTGGACTGTAGAACCATGTC60.2643171806167401No Hit
GTGCGCTTACACATCCCTGAGAACTTTTGCTTGGACTGTAGAACCATGTC60.2643171806167401No Hit
GCTGCGCTTACACATCTCTTCTAAGCTGTGCAGGCTCGATAAGCTGCTTG60.2643171806167401No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAATCAAGTCAAATTTCTT50.22026431718061676No Hit
GCTGCGCTTACACATCTCTGCTAAGCTGCTTGGGGATGACCCCACGAAGC50.22026431718061676No Hit
GCTGCGCTTACACATCTCTGAGAACTTTTGCCGACATCCCTGAGAACTTT50.22026431718061676No Hit
GCTGCGCTTACACATCTCTTCCCTCATCAACACGCCATCCCCTCATCAAC40.1762114537444934No Hit
GCTGCGCTTACACATCTCGACATCCCTGAGAACTTTTGCTTGGACTGTAG40.1762114537444934No Hit
TCCTATCAGTGGCCGCAAAGCCTAAACAGGAGTTTTGGACTGTAGAACCA40.1762114537444934No Hit
GTGCGCTTACACATCTCTGAGAACTTTTGCTTGGACTGTAGAACCATGTC40.1762114537444934No Hit
GCTGCGCTTACACATCTCTGAGAACTTTGCTTGGACTGTAGAACCATGTC40.1762114537444934No Hit
GCTGCGCTTACACATCTCTTCTAAGCTGTGCAGGCTCGATAAGCAGTGGT30.13215859030837004No Hit
GCTGCGCTTACACATCTCTGCTAAGCTGCTTGGGGATGACCGAGTGTGAG30.13215859030837004No Hit
GCTGCGCTTACACATCTCTTCTAAGCTGCTTGGGGATGACCCACGAAGCA30.13215859030837004No Hit
TGCTGATCATTGTTGCTTTGGAGGTGAGGAAAAGTCTGGAGTATTTCCTC30.13215859030837004No Hit
TGTTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC30.13215859030837004No Hit
GCTGCGCTTACACATCTCTGAGCCCACGAGACCCCATGTACTCTGCGTTG30.13215859030837004No Hit
GCTGCGCTTACACATCTCTGCTAAGCTGCTTGGGGATGACCCATGAAGCA30.13215859030837004No Hit
GCTGCGCTTACACATCTCTGACATCCCTGAGAACTTTGCTTGGACTGTAG30.13215859030837004No Hit
GCTGCGTTACACATCTCTGAGAACTTTTGCTTGGACTGTAGAACCATGTC30.13215859030837004No Hit
GCTGCGCTTACACATCCCTGAAACTTTTGCTTGGACTGTAGAACCATGTC30.13215859030837004No Hit
GCTGCGCTTACACATCTCTGACATCCCTAGAACTTTTGCTTGGACTGTAG30.13215859030837004No Hit
GCTGCGCTTACACACCTCTTCCACTTCTTTTACTCTGGCATATTTGAGAC30.13215859030837004No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)