FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008144674

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008144674
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1695
Total Bases245.4 kbp
Sequences flagged as poor quality0
Sequence length141-145
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAAAAGTTCTCAGGGATGTGTAAGCGCAGCGGCTAAAGACCAAGTCTCT49028.908554572271388No Hit
GCAAAAGTTCTCAGAGATGTGTAAGCGCAGCGGCTAAAGACCAAGTCTCT35420.884955752212388No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACAC804.71976401179941No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA774.542772861356932No Hit
GCAAAAGTTCTCAGGGATGTGTAAGCGCAGCCTTCCCATGTACTCTGCGT694.070796460176991No Hit
CGCCAGGCAGGAATCATTCAGGCCTTGCAGAATTTGGGACTCTGCCATAT643.775811209439528No Hit
GCAAAAGTTCTCAGGGATGTCGGCAAAAGTTCTCAGGGATGTAGGCAAAA281.6519174041297935No Hit
AGCAACAATGATCAGCAGGCTAAAGACCAAGTCTCTGCTACCGTAGAGTG231.3569321533923304No Hit
AGCACTTGAATCAACAGGCTAAAGACCAAGTCTCTGCTACCGTAGAGTGA191.12094395280236No Hit
GCAAAAGTTCTCAGGGATGTCAGAGATGTGTAAGCGCAGCGGCTAAAGAC191.12094395280236No Hit
GCAAAAGTTCTCAGGGATGTTGATGAGGGGATCCCATGTACTCTGCGTTG150.8849557522123894No Hit
GCAAAAGTTCTCAGGGATGTCGATGAGGGGATCCCATGTACTCTGCGTTG140.8259587020648967No Hit
CGCCAGGCAGGAATCATTGGTATCAACGCAGAGTACATGGAAGCAGTGGT110.6489675516224189No Hit
GCAAAAGTTCTCAGGGATGTTAGAGATGTGTAAGCGCAGCGGCTAAAGAC110.6489675516224189No Hit
GCAAAAGTTCTCAGGGATGTCGTGTTGATGAGGGGATCCCATGTACTCTG110.6489675516224189No Hit
GCAAAAGTTCTCAGGGATGTCCTCTGCGTTGATACCACTGCTTCCCATGT100.5899705014749262Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCAAAAGTTCTCAGGGATGTCGTGTTGATGAGGGGATGGCGTGTTGATGA100.5899705014749262No Hit
CGCCAGGCAGGAATCATTCACACTGACGACATGGTTCTACAGTCCAAGCA80.471976401179941No Hit
GCAAAGTTCTCAGGGATGTGTAAGCGCAGCGGCTAAAGACCAAGTCTCTG80.471976401179941No Hit
GCAAAAGTTCTCAGGGATGTACTCTGCGTTGATACCACTGCTTCCCATGT70.41297935103244837Clontech Universal Primer Mix Long (96% over 26bp)
GAAAAGTTCTCAGGGATGTGTAAGCGCAGCGGCTAAAGACCAAGTCTCTG70.41297935103244837No Hit
GCAGCTTAGCAGAGATGTGTAAGCGCAGCGGCTAAAGACCAAGTCTCTGC60.35398230088495575No Hit
GCAAAAGTTCTCAGGGATGTGTAAGCGCAGCCTTACATAGACTGCGACGG60.35398230088495575No Hit
GCAAAAGTTCTCAGGGATGTGTAAACGCAGCGGCTAAAGACCAAGTCTCT60.35398230088495575No Hit
GCAAAAGTTCTCAGGGATGTCGATGAGGGGATGGCGTGTTGATGAGGGGA50.2949852507374631No Hit
CGCCAGGCAGGAATCATTGGTATCAACGCAGAGTACATGGGAAGCAGTGG50.2949852507374631No Hit
GCAAAAGTTCTCAGAGATGTGTAACGCAGCGGCTAAAGACCAAGTCTCTG50.2949852507374631No Hit
GCAAAAGTTCTAGGGATGTGTAAGCGCAGCGGCTAAAGACCAAGTCTCTG50.2949852507374631No Hit
GCAAAAGTTCTCAGGGATGTCACACTGACGACATGGTTCTACAGTCCAAG40.2359882005899705No Hit
GCAAAAGTTCTCAGGGATGTGTAAGCGCACGGCTAAAGACCAAGTCTCTG40.2359882005899705No Hit
AACTCCTGTTTAGGCCTTGCGGCCACTGATAGGAGGCTAAAGACCAAGTC40.2359882005899705No Hit
GCAAAAGTTCTCAGAGATGTGTAAGCGCACGGCTAAAGACCAAGTCTCTG40.2359882005899705No Hit
GCAAAAGTTCTCAGGGATGTCCTCTGCGTTGATGAGGGGATGGCGTGTTG40.2359882005899705No Hit
GCAAAAGTTCTCAGGGATGTCGGCAAAAGTTCTCAGAGATGTGTAAGCGC40.2359882005899705No Hit
GCAAAAGTTCTCAGGGATGGCGTGTTGATGAGGGGATGGCGTGTTGATGA40.2359882005899705No Hit
GCAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGC40.2359882005899705No Hit
GCAAAAGTTCTCAGAGATGTGTAAGCGCAGCGGCTAAAGACAAAGTCTCT30.17699115044247787No Hit
GCAAAAGTTCTCAGGGATGTCCCACTGACGACATGGTTCTACAGTCCAAG30.17699115044247787No Hit
GCAAAAGTTTCAGGGATGTGTAAGCGCAGCGGCTAAAGACCAAGTCTCTG30.17699115044247787No Hit
CGCAGGCAGGAATCATTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT30.17699115044247787No Hit
CGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA30.17699115044247787Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAAAAGTTCTCAGGGATGTGTAAGCGCAACGGCTAAAGACCAAGTCTCT30.17699115044247787No Hit
GCAAAAGTTCTCAGGGATGTGTAAGCGCAGCGGCTAAAGACAAAGTCTCT30.17699115044247787No Hit
GCAAAAGTTCTCAGAGATGTGTAAGCGCAACGGCTAAAGACCAAGTCTCT20.11799410029498525No Hit
GCAAAAGTTCTAGGGATGTCGAGATGTGTAAGCGCAGCGGCTAAAGACCA20.11799410029498525No Hit
CAAAAGTTCTCAGGGATGTCGGCAAAAGTTCTCAGGGATGTAGGCAAAAG20.11799410029498525No Hit
GCAAAAGTTCTCAGGGATGTGTAAGCGCAGCCTCCCCATGTACTCTGCGT20.11799410029498525No Hit
GCAAAAGTTCTCAGAGATGTGTAAACGCAGCGGCTAAAGACCAAGTCTCT20.11799410029498525No Hit
GCAAAAGTTCTCAGAGATGTGTAGCGCAGCGGCTAAAGACCAAGTCTCTG20.11799410029498525No Hit
GCAAAAGTTCTCAGGGATGTCGTGTTGATGAGGGGATAGCGTGTTGATGA20.11799410029498525No Hit
GCAAAGTTCTCAGAGATGTGTAAGCGCAGCGGCTAAAGACCAAGTCTCTG20.11799410029498525No Hit
GCAAAAGTTCTCAGGGATGTCAAAGATGTGTAAGCGCAGCGGCTAAAGAC20.11799410029498525No Hit
GCAAAAGTTCTCAGGGATGTCCTCAGAGATGTGTAAGCGCAGCGGCTAAA20.11799410029498525No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACGC20.11799410029498525No Hit
GCAAAGTTCTCAGGGATGTCAGAGATGTGTAAGCGCAGCGGCTAAAGACC20.11799410029498525No Hit
GCAAAAGTTCTCAGGGATGTGTAAGCACAGCGGCTAAAGACCAAGTCTCT20.11799410029498525No Hit
GCAAAAGATCTCAGAGATGTGTAAGCGCAGCGGCTAAAGACCAAGTCTCT20.11799410029498525No Hit
CGCAGGCAGGAATCATTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA20.11799410029498525No Hit
GCAAAAGTTTCAGAGATGTGTAAGCGCAGCGGCTAAAGACCAAGTCTCTG20.11799410029498525No Hit
GCAAAAGTTCTCAGGGATGTTGATGAGGGGATACCCATGTACTCTGCGTT20.11799410029498525No Hit
GCAAAAGTTCTCAGGGATGTAGGCAAAAGTTCTCAGGGATGTATGCAAAA20.11799410029498525No Hit
AGCACTTGAATAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC20.11799410029498525No Hit
GAAAAGTTCTCAGAGATGTGTAAGCGCAGCGGCTAAAGACCAAGTCTCTG20.11799410029498525No Hit
GCAAAAGTTCTCAGGGATGTCAAAAGTTCTCAGAGATGTGTAAGCGCAGC20.11799410029498525No Hit
GCAAAAGTTCTCAGGGATGTCACTGACGACATGGTTCTACAGTCCAAGCA20.11799410029498525No Hit
GCAAAAGTTTTCAGGGATGTGTAAGCGCAGCGGCTAAAGACCAAGTCTCT20.11799410029498525No Hit
GCAAAAGTTCTCAGAGATGTGTAAGCGCAGCCTTCCCATGTACTCTGCGT20.11799410029498525No Hit
GCAAAAGTTCTCAGGGATGTGTAAGCGCAGCCTTAGCAGAGATGTGTAAG20.11799410029498525No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)