FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008144727

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008144727
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19719
Total Bases2.8 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC726436.837567827983165No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT563128.556214818195645No Hit
TGTTGATTCAAGTGCTTTGGACTGTAGAACCATGTCGTCAGTGTAGATCT14757.480095339520259No Hit
TGCTGATCATTGTTGCTTTGGACTGTAGAACCATGTCGTCAGTGTAGATC10815.482022414929763No Hit
GCTGCGCTTACACATCCCTGAGAACTTTTGCTTGGACTGTAGAACCATGT3871.9625741670470105No Hit
GCTGCGCTTACACATCTCTGAGAACTTTTGCTTGGACTGTAGAACCATGT3801.9270754095035245No Hit
GTTGTTTAGAATGAGGAGAGAGGCCCTGGGCTTCACTTACTCTTCATTCA3101.5720878340686646No Hit
TCCTATCAGTGGCCGCAAGGCCTAAACAGGAGTTTTGGACTGTAGAACCA3071.5568740808357422No Hit
GCTGCGCTTACACATCTCTTCCACTTCTTTTACTCTGGCATATTTGAGAC1450.7353314062579238No Hit
CAACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACA1330.6744763933262335No Hit
TCCTATCAGTGGCCGCAAAGCCTAAACAGGAGTTTTGGACTGTAGAACCA820.41584258836655No Hit
TGCTGATCATTGTTGCTTTGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT740.3752725797454232No Hit
TCCTATCAGTGGCCGCAAGGAGGGGAGAGGCCCTGGGCTTCACTTACTCT500.25356255388204274No Hit
TGCTGTCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT410.207921294183275No Hit
TGCTGATCATTGTTGCTTTGGAGGTGAGGAAAAGTCTGGAGTATTTCCTC400.20285004310563415No Hit
TGTTATTCAAGTGCTTTGGACTGTAGAACCATGTCGTCAGTGTAGATCTC370.1876362898727116No Hit
GCTGCGCTTACACATCTCTGACATCCCTGAGAACTTTTGCTTGGACTGTA350.1774937877174299No Hit
TCCTATCAGTGGCCGCAAAGAGGGGGGAGGCCCTGGGCTTCACTTACTCT300.1521375323292256No Hit
TCCTATAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT300.1521375323292256No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATCTCCTC290.14706628125158477No Hit
TGCTGATCATTGTTGTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT280.1419950301739439No Hit
TCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT270.13692377909630307No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAGGTCTGGAGTATTTCCTC260.1318525280186622No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTCCACTTACTCT250.12678127694102137No Hit
TGCTGATCATTGTTGCTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT240.12171002586338049No Hit
TCCTATCAGTGGCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT240.12171002586338049No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAGAGTCTGGAGTATTTCCTC220.11156752370809879No Hit
TGCTGGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCT210.10649627263045794No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGGGGCCCTGGGCTTCACTTACTCT210.10649627263045794No Hit
TGTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT200.10142502155281707No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCT200.10142502155281707No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)