FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008144740

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008144740
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16851
Total Bases2.4 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA553132.822977864815144No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACAC524131.102011750044507No Hit
AGCACTTGAATCAACAGGCTAAAGACCAAGTCTCTGCTACCGTACAGAGC14348.509880719245148No Hit
AGCAACAATGATCAGCAGGCTAAAGACCAAGTCTCTGCTACCGTACAGAG11977.103435997863629No Hit
GCAAAAGTTCTCAGGGATGTGTAAGCGCAGCGGCTAAAGACCAAGTCTCT4172.4746305857219157No Hit
GCAAAAGTTCTCAGAGATGTGTAAGCGCAGCGGCTAAAGACCAAGTCTCT3902.3144027060708563No Hit
AACTCCTGTTTAGGCCTTGCGGCCACTGATAGGAGGCTAAAGACCAAGTC2631.5607382351195775No Hit
AGCACTTGAATAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC1180.700255177734259No Hit
GGTGTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG620.36793068660613615No Hit
AACTCCTGTTTAGGCTTTGCGGCCACTGATAGGAGGCTAAAGACCAAGTC600.35606195478013175No Hit
GGTGTTTGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG570.3382588570411251No Hit
AAGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACA440.2611121001720966No Hit
GGTGTTTGGGATGGATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG430.2551777342590944No Hit
GCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC390.23144027060708564No Hit
GGTGTTTGGGTGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG370.21957153878108127No Hit
GCAAAAGTTCTCAGGGATGTGTAAGCGCAGCCTTCCCATGTACTCTGCGT350.20770280695507687No Hit
GTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG310.18396534330306807No Hit
GCAAAAGTTCTCAGGGATGTCAGAGATGTGTAAGCGCAGCGGCTAAAGAC300.17803097739006588No Hit
AGCACTTGAATCAACAGGCTAAAGACCAAGTCTCTGCTACCGAACAGAGC290.17209661147706368No Hit
AGCACTTGAATAAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACAC260.1542935137380571No Hit
GCAAAAGTTCTCAGGGATGTCGGCAAAAGTTCTCAGGGATGTAGGCAAAA250.1483591478250549No Hit
GGTGTTTGGGAGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG220.1305560500860483No Hit
AGCACTTGAATAACAGGCTAAAGACCAAGTCTCTGCTACCGTACAGAGCT200.1186873182600439No Hit
AGCACTTGAATCACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC200.1186873182600439No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCACAGAAGCAGCA180.10681858643403952No Hit
GGTGTTTGGGATGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG170.10088422052103732No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)