FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008144752

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008144752
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8081
Total Bases1.1 Mbp
Sequences flagged as poor quality0
Sequence length143-145
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA278734.48830590273481No Hit
AACTCCTGTTTAGGCCTTGCAGAATTTGGGACTCTGCCATATTCTTTCCG136516.891473827496597No Hit
AGCACTTGAATCAACAGGCTAAAGACCAAGTCTCTGCTACCGTAACGTCA92311.42185373097389No Hit
AGCAACAATGATCAGCAGGCTAAAGACCAAGTCTCTGCTACCGTAACGTC6918.550921915604505No Hit
GCAAAAGTTCTCAGAGATGTGTAAGCGCAGCGGCTAAAGACCAAGTCTCT3534.368271253557729No Hit
GCAAAAGTTCTCAGGGATGTGTAAGCGCAGCGGCTAAAGACCAAGTCTCT2933.6257888875139215No Hit
AACTCCTGTTTAGGCCTTGCGGCCACTGATAGGAGGCTAAAGACCAAGTC2633.254547704492018No Hit
CGCCAGGCAGGAATCATTCAGGCCTTGCAGAATTTGGGACTCTGCCATAT1151.4230912015839623No Hit
AACTCCTGTTTAGGCTTTGCGGCCACTGATAGGAGGCTAAAGACCAAGTC800.9899764880584087No Hit
GCAAAAGTTCTCAGGGATGTCAGAGATGTGTAAGCGCAGCGGCTAAAGAC280.346491770820443No Hit
ACTCCTGTTTAGGCCTTGCAGAATTTGGGACTCTGCCATATTCTTTCCGT260.3217423586189828No Hit
GCAAAAGTTCTCAGGGATGTGTAAGCGCAGCCTTCCCATGTACTCTGCGT250.3093676525182527No Hit
GGTGTTTGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG210.25986882811533224No Hit
AGCACTTGAATAACAGGCTAAAGACCAAGTCTCTGCTACCGTAACGTCAG210.25986882811533224No Hit
GGTGTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG200.24749412201460216No Hit
AACTCCTGTTAGGCCTTGCAGAATTTGGGACTCTGCCATATTCTTTCCGT190.23511941591387203No Hit
AGCACTTGAATCAACAGGCTAAAGACCAAGTCTCTGCTACCGAAACGTCA150.18562059151095162No Hit
GGTGTTTGGGTGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG150.18562059151095162No Hit
GGTGTTTGGGATGGATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG140.1732458854102215No Hit
GTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG130.1608711793094914No Hit
AACTCTGTTTAGGCCTTGCAGAATTTGGGACTCTGCCATATTCTTTCCGT110.13612176710803117No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCG100.12374706100730108No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGCTCGCAGAAGCAGCA100.12374706100730108No Hit
GCAAAAGTTCTCAGGGATGTCACACTGACGACATGGTTCTACAGTCCAAG90.11137235490657096No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCGGGTGGTTCGCAGAAGCAGCA90.11137235490657096No Hit
AGCAACAATGACAGCAGGCTAAAGACCAAGTCTCTGCTACCGTAACGTCA90.11137235490657096No Hit
AATCCTGTTTAGGCCTTGCAGAATTTGGGACTCTGCCATATTCTTTCCGT90.11137235490657096No Hit
GCAAAGTTCTCAGAGATGTGTAAGCGCAGCGGCTAAAGACCAAGTCTCTG90.11137235490657096No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)