FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008144823

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008144823
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21178
Total Bases3 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC654130.885824912645198No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT495923.415808858249125No Hit
GCTGCGCTTACACATCTCTTCCACTTCTTTTACTCTGGCATATTTGAGAC16587.828879025403721No Hit
TGTTGATTCAAGTGCTTTGGACTGTAGAACCATGTCGTCAGTGTAGATCT14556.870337142317499No Hit
TGCTGATCATTGTTGCTTTGGACTGTAGAACCATGTCGTCAGTGTAGATC10995.18934743601851No Hit
GCTGCGCTTACACATCTCTGAGAACTTTTGCTTGGACTGTAGAACCATGT5252.4789876286712627No Hit
GCTGCGCTTACACATCCCTGAGAACTTTTGCTTGGACTGTAGAACCATGT4552.1484559448484277No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAAATCAAGTCAAATTT3611.7045991122863349No Hit
TCCTATCAGTGGCCGCAAGGCCTAAACAGGAGTTTTGGACTGTAGAACCA3071.449617527623005No Hit
GTTGTTTAGAATGAGGAGAGAGGCCCTGGGCTTCACTTACTCTTCATTCA2341.1049202002077627No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAATCAAGTCAAATTTC1860.8782699027292473No Hit
CAACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACA1080.5099631693266597No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAAAATCAAGTCAAATT1040.49107564453678343No Hit
TCCTATCAGTGGCCGCAAAGCCTAAACAGGAGTTTTGGACTGTAGAACCA740.3494192086127113No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAATCAAGTCAAATTTCT650.30692227783548964No Hit
TGCTGATCATTGTTGCTTTGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT640.3022003966380206No Hit
GCTGCGCTTACAATCTCTTCCACTTCTTTTACTCTGGCATATTTGAGACT580.27386910945320614No Hit
GCTGCGCTTACACATCTCTGACATCCCTGAGAACTTTTGCTTGGACTGTA460.21720653508357732No Hit
TGCTGATCATTGTTGTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT430.20304089149117008No Hit
TGCTGTCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT390.18415336670129379No Hit
TGCTGATCATTGTTGCTTTGGAGGTGAGGAAAAGTCTGGAGTATTTCCTC330.15582207951647936No Hit
TCCTATCAGTGGCCGCAAGGAGGGGAGAGGCCCTGGGCTTCACTTACTCT320.1511001983190103No Hit
TCCTATCAGTGGCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT300.14165643592407215No Hit
TGTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT290.13693455472660307No Hit
TCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT270.12749079233166494No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAGGTCTGGAGTATTTCCTC270.12749079233166494No Hit
GTGCGCTTACACATCTCTTCCACTTCTTTTACTCTGGCATATTTGAGACT260.12276891113419586No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAGAGTCTGGAGTATTTCCTC250.11804702993672678No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAGAAGTCTGGAGTATTTCCTC240.11332514873925773No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTCCACTTACTCT220.10388138634431958No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)