FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008144858

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008144858
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18807
Total Bases2.7 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA619332.92922847875791No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACAC514727.367469559206683No Hit
AGCACTTGAATCAACAGGCTAAAGACCAAGTCTCTGCTACCGTACGTCAC16408.72015738820652No Hit
AGCAACAATGATCAGCAGGCTAAAGACCAAGTCTCTGCTACCGTACGTCA12576.683681607911948No Hit
AACTCCTGTTTAGGCCTTGCAGAATTTGGGACTCTGCCATATTCTTTCCG8884.721646195565481No Hit
GCAAAAGTTCTCAGGGATGTGTAAGCGCAGCGGCTAAAGACCAAGTCTCT3351.781251661615356No Hit
AACTCCTGTTTAGGCCTTGCGGCCACTGATAGGAGGCTAAAGACCAAGTC3301.7546658159196045No Hit
GCAAAAGTTCTCAGAGATGTGTAAGCGCAGCGGCTAAAGACCAAGTCTCT3081.6376880948582977No Hit
CGCCAGGCAGGAATCATTCAGGCCTTGCAGAATTTGGGACTCTGCCATAT1680.8932844153772532No Hit
AGCACTTGAATAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC1300.6912319880895411No Hit
AACTCCTGTTTAGGCTTTGCGGCCACTGATAGGAGGCTAAAGACCAAGTC640.34029882490562025No Hit
GGTGTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG610.3243473174881693No Hit
GGTGTTTGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG530.2818099643749668No Hit
GTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG400.21268676556601265No Hit
AGCACTTGAATAACAGGCTAAAGACCAAGTCTCTGCTACCGTACGTCACA360.1914180890094114No Hit
GGTGTTTGGGATGGATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG340.18078375073111075No Hit
AGCACTTGAATCACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC320.17014941245281012No Hit
AAGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACA310.1648322433136598No Hit
GGTGTTTGGGTGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG270.14356356675705853No Hit
ACTCCTGTTTAGGCCTTGCAGAATTTGGGACTCTGCCATATTCTTTCCGT250.1329292284787579No Hit
AGCACTTGAATAAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACAC250.1329292284787579No Hit
GCAAAAGTTCTCAGGGATGTGTAAGCGCAGCCTTCCCATGTACTCTGCGT250.1329292284787579No Hit
GGTGTTTGGGAGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG230.12229489020045728No Hit
AGCACTTGAATCAACAGGCTAAAGACCAAGTCTCTGCTACCGAACGTCAC210.11166055192215665No Hit
GGTGTTTGGGATGGAATGGTGGTCCACGCAGGTGGTTCGCAGAAGCAGCA210.11166055192215665No Hit
GGTGTTTGGGATGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG210.11166055192215665No Hit
GCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC200.10634338278300633No Hit
GGTTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG200.10634338278300633No Hit
GCAAAAGTTCTCAGGGATGTCAGAGATGTGTAAGCGCAGCGGCTAAAGAC190.10102621364385601No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)