FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008144872

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008144872
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13036
Total Bases1.8 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACAC591545.37434795949678No Hit
AGCACTTGAATCAACAGGCTAAAGACCAAGTCTCTGCTACCGTAACGCAC148411.383860079779073No Hit
AGCAACAATGATCAGCAGGCTAAAGACCAAGTCTCTGCTACCGTAACGCA11238.614605707272169No Hit
AACTCCTGTTTAGGCCTTGCAGAATTTGGGACTCTGCCATATTCTTTCCG11008.438171218165081No Hit
GCAAAAGTTCTCAGAGATGTGTAAGCGCAGCGGCTAAAGACCAAGTCTCT4233.244860386621663No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA4083.1297944154648665No Hit
GCAAAAGTTCTCAGGGATGTGTAAGCGCAGCGGCTAAAGACCAAGTCTCT3322.546793494937097No Hit
AACTCCTGTTTAGGCCTTGCGGCCACTGATAGGAGGCTAAAGACCAAGTC2551.9561215096655415No Hit
AGCACTTGAATAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC1270.9742252224608776No Hit
AACTCCTGTTTAGGCTTTGCGGCCACTGATAGGAGGCTAAAGACCAAGTC600.46026388462718626No Hit
AAGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACA440.33752684872660327No Hit
GCAAAAGTTCTCAGGGATGTGTAAGCGCAGCCTTCCCATGTACTCTGCGT430.3298557839828168No Hit
AGCACTTGAATAACAGGCTAAAGACCAAGTCTCTGCTACCGTAACGCACA320.245474071801166No Hit
ACTCCTGTTTAGGCCTTGCAGAATTTGGGACTCTGCCATATTCTTTCCGT300.23013194231359313No Hit
AGCACTTGAATCACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC300.23013194231359313No Hit
GCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC280.21478981282602025No Hit
GCAAAAGTTCTCAGGGATGTCCAGCAACTCTGCGTTGATACCACTGCTTC270.2071187480822338Clontech SMART CDS Primer II A (95% over 24bp)
AGCACTTGAATAAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACAC250.19177661859466094No Hit
AGCACTTGAATCAACAGGCTAAAGACCAAGTCTCTGCTACCGAAACGCAC250.19177661859466094No Hit
AGCACTTGATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC180.13807916538815587No Hit
AGCACTTGAATCAACAGGTTTCAGAGACTGAGCCATTTTCAAACTCACAC180.13807916538815587No Hit
AGCACTTGAATCAACAGGCTAAAGACCAAGTCTCTGCTACCGTAGCGGCC180.13807916538815587No Hit
AGCACTTGAATCAACAGGCTACAGAGACTGAGCCATTTTCAAACTCACAC170.13040810064436945No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACCCACAC160.122737035900583No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTCCAAACTCACAC150.11506597115679656No Hit
AGCACTTGAATCAACAGGCTTCAGGGACTGAGCCATTTTCAAACTCACAC150.11506597115679656No Hit
AACTCCTGTTAGGCCTTGCAGAATTTGGGACTCTGCCATATTCTTTCCGT150.11506597115679656No Hit
GCAAAAGTTCTCAGGGATGTCAGAGATGTGTAAGCGCAGCGGCTAAAGAC140.10739490641301012No Hit
AGCACTTGAATCAACAGGCTTCAGAGACCGAGCCATTTTCAAACTCACAC140.10739490641301012No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)