FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008144882

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008144882
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19542
Total Bases2.8 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACAC615031.470678538532393No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA460323.55439566062839No Hit
AACTCCTGTTTAGGCCTTGCAGAATTTGGGACTCTGCCATATTCTTTCCG202610.367413775457988No Hit
AGCACTTGAATCAACAGGCTAAAGACCAAGTCTCTGCTACCGTATCACGC15658.00839218094361No Hit
AGCAACAATGATCAGCAGGCTAAAGACCAAGTCTCTGCTACCGTATCACG12426.3555419097328825No Hit
GCAAAAGTTCTCAGAGATGTGTAAGCGCAGCGGCTAAAGACCAAGTCTCT3551.816600143281138No Hit
AACTCCTGTTTAGGCCTTGCGGCCACTGATAGGAGGCTAAAGACCAAGTC3241.6579674547129262No Hit
GCAAAAGTTCTCAGGGATGTGTAAGCGCAGCGGCTAAAGACCAAGTCTCT3131.6016784361887217No Hit
CGCCAGGCAGGAATCATTCAGGCCTTGCAGAATTTGGGACTCTGCCATAT1770.9057414798894688No Hit
AGCACTTGAATAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC1560.7982806263432606No Hit
AACTCCTGTTTAGGCTTTGCGGCCACTGATAGGAGGCTAAAGACCAAGTC740.3786715791628288No Hit
GCAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGC520.2660935421144202No Hit
GGTGTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG460.23539044110121787No Hit
ACTCCTGTTTAGGCCTTGCAGAATTTGGGACTCTGCCATATTCTTTCCGT390.19957015658581514No Hit
AAGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACA370.1893357895814144No Hit
GGTGTTTGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG340.17398423907481322No Hit
GCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC290.14839832156381128No Hit
AACTCCTGTTAGGCCTTGCAGAATTTGGGACTCTGCCATATTCTTTCCGT280.14328113806161089No Hit
GCAAAAGTTCTCAGGGATGTCAGAGATGTGTAAGCGCAGCGGCTAAAGAC280.14328113806161089No Hit
AGCACTTGAATAAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACAC280.14328113806161089No Hit
AGCACTTGAATCAACAGGCTAAAGACCAAGTCTCTGCTACCGAATCACGC270.1381639545594105No Hit
GTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG260.1330467710572101No Hit
AGCACTTGAATCACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC260.1330467710572101No Hit
GGTGTTTGGGTGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG240.12281240405280934No Hit
AGCACTTGAATAACAGGCTAAAGACCAAGTCTCTGCTACCGTATCACGCT230.11769522055060894No Hit
GCAAAAGTTCTCAGGGATGTGTAAGCGCAGCCTTCCCATGTACTCTGCGT230.11769522055060894No Hit
AGCACTTGAATCAACAGGCTACAGAGACTGAGCCATTTTCAAACTCACAC200.10234367004400778No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)