FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008144906

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008144906
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16090
Total Bases2.3 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACAC493930.69608452454941No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA324920.19266625233064No Hit
AACTCCTGTTTAGGCCTTGCAGAATTTGGGACTCTGCCATATTCTTTCCG182011.311373523927907No Hit
AGCACTTGAATCAACAGGCTAAAGACCAAGTCTCTGCTACCGTAGACGTC15459.602237414543195No Hit
AGCAACAATGATCAGCAGGCTAAAGACCAAGTCTCTGCTACCGTAGACGT12097.513983840894965No Hit
GCAAAAGTTCTCAGGGATGTGTAAGCGCAGCGGCTAAAGACCAAGTCTCT3452.1441889372280922No Hit
GCAAAAGTTCTCAGAGATGTGTAAGCGCAGCGGCTAAAGACCAAGTCTCT3272.0323182100683654No Hit
AACTCCTGTTTAGGCCTTGCGGCCACTGATAGGAGGCTAAAGACCAAGTC2971.845866998135488No Hit
AGCACTTGAATAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC1300.8079552517091362No Hit
CGCCAGGCAGGAATCATTCAGGCCTTGCAGAATTTGGGACTCTGCCATAT650.4039776258545681No Hit
AACTCCTGTTTAGGCTTTGCGGCCACTGATAGGAGGCTAAAGACCAAGTC570.35425730267246736No Hit
ACTCCTGTTTAGGCCTTGCAGAATTTGGGACTCTGCCATATTCTTTCCGT380.23617153511497824No Hit
AGCACTTGAATAACAGGCTAAAGACCAAGTCTCTGCTACCGTAGACGTCT360.22374145431945305No Hit
GGTGTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG350.2175264139216905No Hit
AAGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACA340.2113113735239279No Hit
GCAAAAGTTCTCAGGGATGTGTAAGCGCAGCCTTCCCATGTACTCTGCGT310.19266625233064014No Hit
GGTGTTTGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG300.18645121193287756No Hit
GCAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTG290.18023617153511498No Hit
GCAAAAGTTCTCAGGGATGTCAGAGATGTGTAAGCGCAGCGGCTAAAGAC280.1740211311373524No Hit
GCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC270.1678060907395898No Hit
AACTCCTGTTAGGCCTTGCAGAATTTGGGACTCTGCCATATTCTTTCCGT250.15537600994406464No Hit
AGCACTTGAATCACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC240.14916096954630206No Hit
GGTGTTTGGGATGGAATGGTGATCCACACAGGTGGTTCGCAGAAGCAGCA200.1243008079552517No Hit
GGTGTTTGGGATGGATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG190.11808576755748912No Hit
AGCACTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC170.10565568676196395No Hit
AGCACTTGAATCAACAGGTTTCAGAGACTGAGCCATTTTCAAACTCACAC170.10565568676196395No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)