FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008144937

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008144937
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4435
Total Bases642.5 kbp
Sequences flagged as poor quality0
Sequence length140-145
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTGATTCAAGTGCTTTGGACTGTAGAACCATGTCGTCAGTGTAGATCT87119.63923337091319No Hit
TGCTGATCATTGTTGCTTTGGACTGTAGAACCATGTCGTCAGTGTAGATC64314.498308906426155No Hit
GCTGCGCTTACACATCTCTGAGAACTTTTGCTTGGACTGTAGAACCATGT44610.056369785794814No Hit
GCTGCGCTTACACATCTCTTCCACTTCTTTTACTCTGGCATATTTGAGAC3658.229988726042842No Hit
GCTGCGCTTACACATCCCTGAGAACTTTTGCTTGGACTGTAGAACCATGT3507.891770011273957No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAATCAAGTCAAATTTC2776.245772266065389No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAATCAAGTCAAATTTCT1643.697857948139797No Hit
TCCTATCAGTGGCCGCAAGGCCTAAACAGGAGTTTTGGACTGTAGAACCA1593.5851183765501693No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC1413.1792559188275087No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAAATCAAGTCAAATTT1052.367531003382187No Hit
TCCTATCAGTGGCCGCAAAGCCTAAACAGGAGTTTTGGACTGTAGAACCA461.0372040586245772No Hit
GCTGCGCTTACACATCTCTTCTAAGCAGTGGTATCAACGCAGAGTACATG350.7891770011273956Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAATCAAGTCAAATTTCTT340.7666290868094702No Hit
TGTTATTCAAGTGCTTTGGACTGTAGAACCATGTCGTCAGTGTAGATCTC180.4058624577226606No Hit
GCTGCGCTTACACATCTCTGCTAAGCAGTGGTATCAACGCAGAGTACATG170.3833145434047351Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTGCGCTTACACATCTCTGACATCCCTGAGAACTTTTGCTTGGACTGTA150.3382187147688839No Hit
GCTGCGCTTACACATCTCGGACATCCCTGAGAACTTTTGCTTGGACTGTA140.3156708004509583No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAAAATCAAGTCAAATT120.2705749718151071No Hit
GCTGCGCTTACACATCCCTGAGAACTTTGCTTGGACTGTAGAACCATGTC90.2029312288613303No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT90.2029312288613303No Hit
TGTTGATTCAAGTGCTTTTGGACTGTAGAACCATGTCGTCAGTGTAGATC80.18038331454340473No Hit
GCTGCGCTTACAATCTCTTCCACTTCTTTTACTCTGGCATATTTGAGACT70.15783540022547915No Hit
GCTGCGTTTACACATCTCTGAGAACTTTTGCTTGGACTGTAGAACCATGT60.13528748590755355No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAATCAAGTCAAATTTA60.13528748590755355No Hit
GCTGCGCTTACACATCCTGAGAACTTTTGCTTGGACTGTAGAACCATGTC50.11273957158962795No Hit
TGCTGATCATTGTTGCTTTGGGCTGTAGAACCATGTCGTCAGTGTGTTGA50.11273957158962795No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAATCAAGTCAAATTTCTTA50.11273957158962795No Hit
GTGCGCTTACACATCTCTTCCACTTCTTTTACTCTGGCATATTTGAGACT50.11273957158962795No Hit
TGTGATTCAAGTGCTTTGGACTGTAGAACCATGTCGTCAGTGTAGATCTC50.11273957158962795No Hit
GTTGTTTAGAATGAGAGAGAGGCAAAAAAAAAAAATCAAGTCAAATTTCT50.11273957158962795No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAATAAAGTCAAATTTC50.11273957158962795No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)