FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008144995

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008144995
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10025
Total Bases1.4 Mbp
Sequences flagged as poor quality0
Sequence length139-145
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC454045.28678304239401No Hit
TGTTGATTCAAGTGCTTTGGACTGTAGAACCATGTCGTCAGTGTAGATCT153615.321695760598503No Hit
TGCTGATCATTGTTGCTTTGGACTGTAGAACCATGTCGTCAGTGTAGATC118911.860349127182044No Hit
GCTGCGCTTACACATCCCTGAGAACTTTTGCTTGGACTGTAGAACCATGT4484.468827930174563No Hit
GCTGCGCTTACACATCTCTGAGAACTTTTGCTTGGACTGTAGAACCATGT3763.7506234413965083No Hit
TCCTATCAGTGGCCGCAAGGCCTAAACAGGAGTTTTGGACTGTAGAACCA2132.1246882793017456No Hit
TCCTATCAGTGGCCGCAAAGCCTAAACAGGAGTTTTGGACTGTAGAACCA600.5985037406483791No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT600.5985037406483791No Hit
TGCTGATCATTGTTGCTTTGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT430.428927680798005No Hit
GCTGCGCTTACACATCTCTGCTAAGCTGCTTGGGGATGACCCGAAGCAGT360.35910224438902744No Hit
TGCTGATCATTGTTGCTTTGGAGGTGAGGAAAAGTCTGGAGTATTTCCTC350.3491271820448878No Hit
GCTGCGCTTACACATCTCTGAGAACTTTTGCCTACATCCCTGAGAACTTT300.29925187032418954No Hit
CAACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACA280.2793017456359102No Hit
TGTTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC280.2793017456359102No Hit
TGCTGATCATTGTTGTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT270.26932668329177056No Hit
TGTTATTCAAGTGCTTTGGACTGTAGAACCATGTCGTCAGTGTAGATCTC230.22942643391521197No Hit
GCTGCGCTTACACATCTCTGCTAAGCTGTGCAGGCTCGATAAGCTGCTTG220.21945137157107233No Hit
TGCTGTCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT220.21945137157107233No Hit
TGCTGATCATTGTTGCTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT200.199501246882793No Hit
GCTGCGCTTACACATCTCTGACATCCCTGAGAACTTTTGCTTGGACTGTA190.18952618453865336No Hit
GCTGCGCTTACACATCTCTTCCACTTCTTTTACTCTGGCATATTTGAGAC160.15960099750623444No Hit
GCTGCGCTTACACATCTCTGCTAAGCTGCTTGGGGATGACCCCACGAAGC160.15960099750623444No Hit
TGTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT160.15960099750623444No Hit
GCTGCGCTTACACATCTCTTCTAAGCAGTGGTATCAACGCAGAGTACATG140.1396508728179551Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTGCGCTTACACATCCCTGAGAACTTTTGCCTACATCCCTGAGAACTTT130.12967581047381546No Hit
TGCTGGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCT110.10972568578553617No Hit
TGTTGATCATTGTTGCTTTGGACTGTAGAACCATGTCGTCAGTGTAGATC110.10972568578553617No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)