FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008145023

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008145023
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3179
Total Bases460.9 kbp
Sequences flagged as poor quality0
Sequence length143-145
%GC64

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTGATTCAAGTGCTTTGGACTGTAGAACCATGTCGTCAGTGTAGATCT102732.305756527209816No Hit
TGCTGATCATTGTTGCTTTGGACTGTAGAACCATGTCGTCAGTGTAGATC66720.981440704624095No Hit
GCTGCGCTTACACATCCCTGAGAACTTTTGCTTGGACTGTAGAACCATGT36911.607423718150361No Hit
GCTGCGCTTACACATCTCTGAGAACTTTTGCTTGGACTGTAGAACCATGT2818.839257628184964No Hit
TCCTATCAGTGGCCGCAAGGCCTAAACAGGAGTTTTGGACTGTAGAACCA2056.448568732305756No Hit
TCCTATCAGTGGCCGCAAAGCCTAAACAGGAGTTTTGGACTGTAGAACCA491.5413652091852783No Hit
TGTTATTCAAGTGCTTTGGACTGTAGAACCATGTCGTCAGTGTAGATCTC210.6605850896508336No Hit
GCTGCGCTTACACATCTCTGACATCCCTGAGAACTTTTGCTTGGACTGTA200.6291286568103177No Hit
GCTGCGCTTACACATCCCTGAGAACTTTGCTTGGACTGTAGAACCATGTC90.28310789556464294No Hit
TGCTGATCATTGTTGCTTTGGACTGTAGAACCATGTCGTCAGTGTGTTGA70.2201950298836112No Hit
TGTTGATTCAAGTGCTTTTGGACTGTAGAACCATGTCGTCAGTGTAGATC70.2201950298836112No Hit
TCCTATCAGTGGCCGCAAGGCCTAACAGGAGTTTTGGACTGTAGAACCAT60.1887385970430953No Hit
TGTTGATTCAAGTGCTTGGACTGTAGAACCATGTCGTCAGTGTAGATCTC60.1887385970430953No Hit
TGTTGATCAAGTGCTTTGGACTGTAGAACCATGTCGTCAGTGTAGATCTC60.1887385970430953No Hit
GCTGCGCTTACACATCTCTGCTAAGCAGTGGTATCAACGCAGAGTACATG60.1887385970430953Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTGCGCTTACACATCTCTAACATCCCTGAGAACTTTTGCTTGGACTGTA60.1887385970430953No Hit
GCTGCGCTTACACATCCCTAGAACTTTTGCTTGGACTGTAGAACCATGTC60.1887385970430953No Hit
GCTGCGCTTACACATCTCTTCTAAGCAGTGGTATCAACGCAGAGTACATG60.1887385970430953Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCTGATCATTGTTGCTTTGGACTGTAGAACCATGTCGTCAGTGTTGATT60.1887385970430953No Hit
TGCTGATCATTGTTGTTTTGGACTGTAGAACCATGTCGTCAGTGTAGATC50.15728216420257943No Hit
GTTGTTTAGAATGAGGAGAGAGGGTAGCAGAGACTTGGTCTTTAGCCTGT50.15728216420257943No Hit
GTTGCGCTTACACATCCCTGAGAACTTTTGCTTGGACTGTAGAACCATGT50.15728216420257943No Hit
TCCTATCAGTGGCCGCAAGGCCTAAACAGGAGTTTGGACTGTAGAACCAT50.15728216420257943No Hit
TGCTGTCATTGTTGCTTTGGACTGTAGAACCATGTCGTCAGTGTAGATCT50.15728216420257943No Hit
TGTTGATTCAAGTGCTTTGGACTGTAGAACCATGTCGTCAGTGTTGATTC50.15728216420257943No Hit
TCCTATCAGTGGCCGCAAGGCCTAAACAGAGTTTTGGACTGTAGAACCAT40.12582573136206354No Hit
TGTTGATTCAAGTGCTTTGGACTGTAGAACCATGTCGTCCAGTGTAGATC40.12582573136206354No Hit
GTGCGCTTACACATCTCTGAGAACTTTTGCTTGGACTGTAGAACCATGTC40.12582573136206354No Hit
TGCTGATCATTGTGCTTTGGACTGTAGAACCATGTCGTCAGTGTAGATCT40.12582573136206354No Hit
TCCTTAAAATTGAAGCATTGGACTGTAGAACCATGTCGTCAGTGTAGATC40.12582573136206354No Hit
GCTGCGTTTACACATCCCTGAGAACTTTTGCTTGGACTGTAGAACCATGT40.12582573136206354No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)