FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008145024

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008145024
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2930
Total Bases424.7 kbp
Sequences flagged as poor quality0
Sequence length142-145
%GC60

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACTTGAATCAACAGGCTAAAGACCAAGTCTCTGCTACCGTAATCGCA76926.245733788395903No Hit
AGCAACAATGATCAGCAGGCTAAAGACCAAGTCTCTGCTACCGTAATCGC63721.74061433447099No Hit
GCAAAAGTTCTCAGGGATGTGTAAGCGCAGCGGCTAAAGACCAAGTCTCT31110.61433447098976No Hit
GCAAAAGTTCTCAGAGATGTGTAAGCGCAGCGGCTAAAGACCAAGTCTCT30710.477815699658704No Hit
AACTCCTGTTTAGGCCTTGCGGCCACTGATAGGAGGCTAAAGACCAAGTC1946.621160409556313No Hit
AACTCCTGTTTAGGCTTTGCGGCCACTGATAGGAGGCTAAAGACCAAGTC491.6723549488054608No Hit
GCAAAAGTTCTCAGGGATGTCAGAGATGTGTAAGCGCAGCGGCTAAAGAC331.1262798634812288No Hit
AGCACTTGAATAACAGGCTAAAGACCAAGTCTCTGCTACCGTAATCGCAT240.8191126279863481No Hit
GCAAAAGTTCTCAGGGATGTGTAAGCGCAGCCTTCCCATGTACTCTGCGT240.8191126279863481No Hit
CGCCAGGCAGGAATCATTCACACTGACGACATGGTTCTACAGTCCAAGCA190.6484641638225256No Hit
AGCACTTGAATCAACAGGCTAAAGACCAAGTCTCTGCTACCGAAATCGCA140.477815699658703No Hit
GCAAAGTTCTCAGAGATGTGTAAGCGCAGCGGCTAAAGACCAAGTCTCTG100.3412969283276451No Hit
AAGCACTTGAATCAACAGGCTAAAGACCAAGTCTCTGCTACCGTAATCGC90.3071672354948805No Hit
GCAAAGTTCTCAGGGATGTGTAAGCGCAGCGGCTAAAGACCAAGTCTCTG90.3071672354948805No Hit
AGCACTTGAATCAACACTGACGACATGGTTCTACAGTCCAAAGCAACAAT80.27303754266211605No Hit
AGCACTTGATCAACAGGCTAAAGACCAAGTCTCTGCTACCGTAATCGCAT80.27303754266211605No Hit
AACTCCTGTTTAGGCCACTGATAGGAGGCTAAAGACCAAGTCTCTGCTAC80.27303754266211605No Hit
GCAAAAGTTCTCAGGGATGTCCGAGATGTGTAAGCGCAGCGGCTAAAGAC70.2389078498293515No Hit
GCAAAAGTTCTCAGGGATGTTAGAGATGTGTAAGCGCAGCGGCTAAAGAC70.2389078498293515No Hit
AGCAACAATGATCAGCAGGCTAAAGACCAAGTCTCTGCTACAGTAATCGC70.2389078498293515No Hit
AGCACTTGAACAACAGGCTAAAGACCAAGTCTCTGCTACCGTAATCGCAT60.20477815699658702No Hit
AGCACCAATGATCAGCAGGCTAAAGACCAAGTCTCTGCTACCGTAATCGC60.20477815699658702No Hit
GCACTTGAATCAACAGGCTAAAGACCAAGTCTCTGCTACCGTAATCGCAT60.20477815699658702No Hit
AGCAACAATGATCACAGGCTAAAGACCAAGTCTCTGCTACCGTAATCGCA60.20477815699658702No Hit
AACTCCTGTTTAGGCCTTGCGCCACTGATAGGAGGCTAAAGACCAAGTCT50.17064846416382254No Hit
GCAAAAGTTCTCAGAGATGTGTAACGCAGCGGCTAAAGACCAAGTCTCTG50.17064846416382254No Hit
AGCAACAATGATCAGCCGGCTAAAGACCAAGTCTCTGCTACCGTAATCGC50.17064846416382254No Hit
ACTCCTGTTTAGGCCTTGCGGCCACTGATAGGAGGCTAAAGACCAAGTCT50.17064846416382254No Hit
GCAAAAGTTCTCAGAGATGTGTAAGCGCAACGGCTAAAGACCAAGTCTCT40.13651877133105803No Hit
GCAAAAGTTCTCAGGATGTGTAAGCGCAGCGGCTAAAGACCAAGTCTCTG40.13651877133105803No Hit
GCAAAAGTTTCAGGGATGTGTAAGCGCAGCGGCTAAAGACCAAGTCTCTG40.13651877133105803No Hit
GCAAAAGTTCTCAGGGATGTGTAAGCGCAGCCTCCCCATGTACTCTGCGT40.13651877133105803No Hit
GCAAAAGTTCTCAGAGATGTGTAAGCGCACGGCTAAAGACCAAGTCTCTG40.13651877133105803No Hit
AGCACTTGAATCAACACACTGACGACATGGTTCTACAGTCCAAAGCAACA40.13651877133105803No Hit
GCAAAAGTTCTCAGGGATGTCGATGAGGGGATCCCATGTACTCTGCGTTG40.13651877133105803No Hit
AGCACTTGAATCAACACACTGACGACATGGTTCTACAGTCCAAAGCACTT40.13651877133105803No Hit
GCAAAAGTTCTCAGGGATGTGTAAGCGCAGCGGCTAAAGACAAAGTCTCT40.13651877133105803No Hit
AGCACTTGAATCAACAGGCTAAAGACCAAGTCTCTGCTACCGTTTGCGGC40.13651877133105803No Hit
GCAAAAGTTCTCAGGGATGTGTAAGCGCACGGCTAAAGACCAAGTCTCTG30.10238907849829351No Hit
AACTCCTGTTTAGGCCTTTCGGCCACTGATAGGAGGCTAAAGACCAAGTC30.10238907849829351No Hit
AGCACTTGAATCACAGGCTAAAGACCAAGTCTCTGCTACCGTAATCGCAT30.10238907849829351No Hit
AGCACTTGAATCAACAGGCTAAAGACCAAGTCTCTGCTACCGTTGCGGCC30.10238907849829351No Hit
AGCACTTGAATCAACAGGCTAAAGACAAAGTCTCTGCTACCGTAATCGCA30.10238907849829351No Hit
AACTCCTGTTTAGGCCTCTCTCCTCATTCTAAACAACGGCTAAAGACCAA30.10238907849829351No Hit
AGCACTTGAATCAACAGGCTAAAGACCAAGTCTCTGCTACCGAAATCCCA30.10238907849829351No Hit
AACTCCTGTTTAGGCCTTGCGGCCATGATAGGAGGCTAAAGACCAAGTCT30.10238907849829351No Hit
GCAAAAGTTCTCAGGGATGTGTAAGCGCAACGGCTAAAGACCAAGTCTCT30.10238907849829351No Hit
AGCACAATGATCAGCAGGCTAAAGACCAAGTCTCTGCTACCGTAATCGCA30.10238907849829351No Hit
AGCACTTGAATCAACAGGCTAAAGACCAAGTCTCTGCTACCCCAAAGCAA30.10238907849829351No Hit
AGCAACAATGATCCAGCAGGCTAAAGACCAAGTCTCTGCTACCGTAATCG30.10238907849829351No Hit
AGCACTTGAATCAACACTGACGACATGGTTCTACAGCCCAAAGCAACAAT30.10238907849829351No Hit
AACTCCTGTTTAGGCCTTGCACTGACGACATGGTTCTACAGTCCAAAGCA30.10238907849829351No Hit
GCAAAAGTTTTCAGGGATGTGTAAGCGCAGCGGCTAAAGACCAAGTCTCT30.10238907849829351No Hit
CGCCAGGCAGGAATCACACTGACGACATGGTTCTACAGTCCAAGCAAAAG30.10238907849829351No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)