FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008145041

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008145041
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3732
Total Bases540.2 kbp
Sequences flagged as poor quality0
Sequence length140-145
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC57615.434083601286176No Hit
GCTGCGCTTACACATCTCTGAGAACTTTTGCTTGGACTGTAGAACCATGT48312.942122186495178No Hit
GCTGCGCTTACACATCCCTGAGAACTTTTGCTTGGACTGTAGAACCATGT45212.111468381564846No Hit
CAACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACA3689.860664523043944No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT3679.833869239013932No Hit
GCTGCGCTTACACATCTCTTCCACTTCTTTTACTCTGGCATATTTGAGAC2917.797427652733119No Hit
TGTTGATTCAAGTGCTTTGGACTGTAGAACCATGTCGTCAGTGTAGATCT1323.536977491961415No Hit
TGCTGATCATTGTTGCTTTGGACTGTAGAACCATGTCGTCAGTGTAGATC932.491961414790997No Hit
GCTGCGCTTACACATCTCTGACATCCCTGAGAACTTTTGCTTGGACTGTA591.5809217577706325No Hit
GCTGCGCTTACACATCTCTTCTAAGCAGTGGTATCAACGCAGAGTACATG471.2593783494105038Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCTATCAGTGGCCGCAAGGCCTAAACAGGAGTTTTGGACTGTAGAACCA391.045016077170418No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCATC381.0182207931404073No Hit
GCTGCGCTTACACATCTCGGACATCCCTGAGAACTTTTGCTTGGACTGTA300.8038585209003215No Hit
GTTGTTTAGAATGAGGAGAGAGGCCCTGGGCTTCACTTACTCTTCATTCA250.669882100750268No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAAATCAAGTCAAATTT210.5627009646302251No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAATCAAGTCAAATTTC180.482315112540193No Hit
GCTGCGCTTACAATCTCTTCCACTTCTTTTACTCTGGCATATTTGAGACT160.4287245444801715No Hit
GCTGCGCTTACACATCTCTGCTAAGCTGTGCAGGCTCGATAAGCTGCTTG130.34833869239013937No Hit
CAACCAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG120.3215434083601286No Hit
GCTGCGCTTACACATCTCTGAGAACTTTGCTTGGACTGTAGAACCATGTC110.29474812433011793No Hit
GCTGCGCTTACACATCTCTGCTAAGCAGTGGTATCAACGCAGAGTACATG100.2679528403001072Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTGCGCTTACACATCCCTGAGAACTTTGCTTGGACTGTAGAACCATGTC90.2411575562700965No Hit
GCTGCGCTTACACATCTCTGCTAAGCTGCTTGGGGATGACCGAGTGTGAG90.2411575562700965No Hit
TCCTATCAGTGGCCGCAAAGCCTAAACAGGAGTTTTGGACTGTAGAACCA90.2411575562700965No Hit
CAACCAAAAGAGAAGGAGGCCCCACAGGTTTCTGGATCCAGCCCCTGACA70.18756698821007503No Hit
GTGCGCTTACACATCTCTTCCACTTCTTTTACTCTGGCATATTTGAGACT60.1607717041800643No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAAAATCAAGTCAAATT60.1607717041800643No Hit
CAACCAAAAGAGAAGGAGGCCATCCCTGAGAACTTTTGCTTGGACTGTAG50.1339764201500536No Hit
TCCTATCAGTGGCCGCAAGGAGGGGAGAGGCCCTGGGCTTCACTTACTCT50.1339764201500536No Hit
TCCTATCAGTGGCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT50.1339764201500536No Hit
TGCTGATCATTGTTGCTTTGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT50.1339764201500536No Hit
GCTGCGCTTACACATCTCTTCCAAGCAGTGGTATCAACGCAGAGTACATG50.1339764201500536Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGGCA50.1339764201500536No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAATCAAGTCAAATTTCT50.1339764201500536No Hit
GGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC50.1339764201500536No Hit
TGCTGATTCAAGTGCTTTGGACTGTAGAACCATGTCGTCAGTGTAGATCT40.10718113612004287No Hit
GCTGCGTTTACACATCTCTTCCACTTCTTTTACTCTGGCATATTTGAGAC40.10718113612004287No Hit
CAACAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG40.10718113612004287No Hit
GCTGCGCTTACACATCTCTGAGAACTTTTCTTGGACTGTAGAACCATGTC40.10718113612004287No Hit
TGCTGATCATTGTTGCTTTGGGGGTGGGGAAAAGTCTGGAGTATTTCCTC40.10718113612004287No Hit
GTGCGCTTACACATCCCTGAGAACTTTTGCTTGGACTGTAGAACCATGTC40.10718113612004287No Hit
GCTGCGCTTACATATCTCTTCCACTTCTTTTACTCTGGCATATTTGAGAC40.10718113612004287No Hit
GTGCGCTTACACATCTCTGAGAACTTTTGCTTGGACTGTAGAACCATGTC40.10718113612004287No Hit
GCTGCGCTTACACATCTCTAACATCCCTGAGAACTTTTGCTTGGACTGTA40.10718113612004287No Hit
GCTGCGCTTACACATCTCTTCTAAGCTGTGCAGGCTCGATAAGCTGCTTG40.10718113612004287No Hit
GCTGCGCTTACACATCCCTAGAACTTTTGCTTGGACTGTAGAACCATGTC40.10718113612004287No Hit
GCTGCGTTTACACATCCCTGAGAACTTTTGCTTGGACTGTAGAACCATGT40.10718113612004287No Hit
TGCGGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC40.10718113612004287No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)