FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008145056

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008145056
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8515
Total Bases1.2 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA245128.784497944803288No Hit
AACTCCTGTTTAGGCCTTGCAGAATTTGGGACTCTGCCATATTCTTTCCG178921.009982384028188No Hit
AGCACTTGAATCAACAGGCTAAAGACCAAGTCTCTGCTACCGTAGTGTTC98511.567821491485613No Hit
AGCAACAATGATCAGCAGGCTAAAGACCAAGTCTCTGCTACCGTAGTGTT7809.16030534351145No Hit
GCAAAAGTTCTCAGGGATGTGTAAGCGCAGCGGCTAAAGACCAAGTCTCT4455.226071638285379No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACAC3684.321785085143864No Hit
GCAAAAGTTCTCAGAGATGTGTAAGCGCAGCGGCTAAAGACCAAGTCTCT3584.204345273047563No Hit
AACTCCTGTTTAGGCCTTGCGGCCACTGATAGGAGGCTAAAGACCAAGTC1932.2665883734586023No Hit
AACTCCTGTTTAGGCTTTGCGGCCACTGATAGGAGGCTAAAGACCAAGTC520.6106870229007634No Hit
GCAAAAGTTCTCAGGGATGTCAGAGATGTGTAAGCGCAGCGGCTAAAGAC350.4110393423370522No Hit
GGTGTTTGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG340.3992953611274222No Hit
ACTCCTGTTTAGGCCTTGCAGAATTTGGGACTCTGCCATATTCTTTCCGT340.3992953611274222No Hit
GGTGTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG270.31708749266001174No Hit
AGCACTTGAATCAACAGGCTAAAGACCAAGTCTCTGCTACCGTAGTGTTA240.28185554903112153No Hit
GGTGTTTGGGATGGATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG160.18790369935408102No Hit
AGCACTTGAATCAACAGGCTAAAGACCAAGTCTCTGCTACCGAAGTGTTC150.17615971814445097No Hit
AACTCTGTTTAGGCCTTGCAGAATTTGGGACTCTGCCATATTCTTTCCGT150.17615971814445097No Hit
GTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG140.1644157369348209No Hit
AACTCCTGTTAGGCCTTGCAGAATTTGGGACTCTGCCATATTCTTTCCGT130.15267175572519084No Hit
GCAAAGTTCTCAGGGATGTGTAAGCGCAGCGGCTAAAGACCAAGTCTCTG110.1291837933059307No Hit
AGCACTTGAATAACAGGCTAAAGACCAAGTCTCTGCTACCGTAGTGTTCG110.1291837933059307No Hit
AACTCCTGGTTTAGGCCTTGCAGAATTTGGGACTCTGCCATATTCTTTCC100.11743981209630064No Hit
AATCCTGTTTAGGCCTTGCAGAATTTGGGACTCTGCCATATTCTTTCCGT100.11743981209630064No Hit
GCAAAAGTTCTCAGGGATGTCCGAGATGTGTAAGCGCAGCGGCTAAAGAC90.10569583088667059No Hit
GCAAAAGTTCTCAGGGATGTTAGAGATGTGTAAGCGCAGCGGCTAAAGAC90.10569583088667059No Hit
AACTCCTGTTTGGCCTTGCAGAATTTGGGACTCTGCCATATTCTTTCCGT90.10569583088667059No Hit
GGTTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG90.10569583088667059No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)