FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008145057

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008145057
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8515
Total Bases1.2 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT242528.479154433352903No Hit
TGTTGATTCAAGTGCTTTGGACTGTAGAACCATGTCGTCAGTGTAGATCT103212.119788608338226No Hit
TGCTGATCATTGTTGCTTTGGACTGTAGAACCATGTCGTCAGTGTAGATC7939.31297709923664No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAAATCAAGTCAAATTT6918.115091015854373No Hit
GCTGCGCTTACACATCCCTGAGAACTTTTGCTTGGACTGTAGAACCATGT4685.4961832061068705No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAATCAAGTCAAATTTC3924.603640634174985No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC3704.345273047563124No Hit
GCTGCGCTTACACATCTCTGAGAACTTTTGCTTGGACTGTAGAACCATGT3664.298297122724604No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAAAATCAAGTCAAATT2803.2883147386964175No Hit
TCCTATCAGTGGCCGCAAGGCCTAAACAGGAGTTTTGGACTGTAGAACCA1922.2548443922489723No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAATCAAGTCAAATTTCT1121.3153258954785672No Hit
TCCTATCAGTGGCCGCAAAGCCTAAACAGGAGTTTTGGACTGTAGAACCA530.6224310041103934No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAAAAATCAAGTCAAAT390.45801526717557256No Hit
GCTGCGCTTACACATCTCTGACATCCCTGAGAACTTTTGCTTGGACTGTA350.4110393423370522No Hit
GTTGTTTAGAATGAGGAGAGAGGCCCTGGGCTTCACTTACTCTTCATTCA290.3405754550792719No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAATCAAGTCAAATTTCTT250.2935995302407516No Hit
TCCTATCAGTGGCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT220.2583675866118614No Hit
TCCTATCAGTGGCCGCAAGGAGGGGAGAGGCCCTGGGCTTCACTTACTCT210.24662360540223136No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAAAATCAAGTAAAATT140.1644157369348209No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAATAAAGTCAAATTTC140.1644157369348209No Hit
TGTTATTCAAGTGCTTTGGACTGTAGAACCATGTCGTCAGTGTAGATCTC120.14092777451556077No Hit
GCTGCGCTTACACATCCCTGAGAACTTTGCTTGGACTGTAGAACCATGTC110.1291837933059307No Hit
TCCTATCATGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT100.11743981209630064No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAAATAAAGTCAAATTT100.11743981209630064No Hit
GCTGCGCTTACACATCTCTTCTAAGCAGTGGTATCAACGCAGAGTACATG100.11743981209630064Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTGCGCTTACACATCTCGGACATCCCTGAGAACTTTTGCTTGGACTGTA90.10569583088667059No Hit
TCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT90.10569583088667059No Hit
GTTGTTTAGAATGAGAGAGAGGCAAAAAAAAAAAATCAAGTCAAATTTCT90.10569583088667059No Hit
GCTGCGCTTACACATCTCTAACATCCCTGAGAACTTTTGCTTGGACTGTA90.10569583088667059No Hit
TCCTTCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT90.10569583088667059No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)