FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008145058

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008145058
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6831
Total Bases989 kbp
Sequences flagged as poor quality0
Sequence length143-145
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCCAGGCAGGAATCATTCAGGCCTTGCAGAATTTGGGACTCTGCCATAT277040.55043185477968No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACAC105315.41501976284585No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA6539.559361733274777No Hit
GCAAAAGTTCTCAGAGATGTGTAAGCGCAGCGGCTAAAGACCAAGTCTCT5137.5098814229249005No Hit
GCAAAAGTTCTCAGGGATGTGTAAGCGCAGCGGCTAAAGACCAAGTCTCT4025.884936319718928No Hit
AGCACTTGAATCAACAGGCTAAAGACCAAGTCTCTGCTACCGTAGGTCAG1782.6057678231591277No Hit
AGCAACAATGATCAGCAGGCTAAAGACCAAGTCTCTGCTACCGTAGGTCA1472.151954325867369No Hit
AACTCCTGTTTAGGCCTTGCGGCCACTGATAGGAGGCTAAAGACCAAGTC510.7465963987703118No Hit
GCAAAAGTTCTCAGGGATGTCAGAGATGTGTAAGCGCAGCGGCTAAAGAC410.6002049480310351No Hit
AGCACTTGAATAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC340.49773093251354117No Hit
CGCAGGCAGGAATCATTCAGGCCTTGCAGAATTTGGGACTCTGCCATATT310.4538134972917582No Hit
GCCAGGCAGGAATCATTCAGGCCTTGCAGAATTTGGGACTCTGCCATATT230.33670033670033667No Hit
AACTCCTGTTTAGGCCTTGCAGAATTTGGGACTCTGCCATATTCTTTCCG190.27814375640462596No Hit
GCAAAAGTTCTCAGGGATGTGTAAGCGCAGCCTTCCCATGTACTCTGCGT190.27814375640462596No Hit
CGCCAGCAGGAATCATTCAGGCCTTGCAGAATTTGGGACTCTGCCATATT170.24886546625677058No Hit
CGCCGGCAGGAATCATTCAGGCCTTGCAGAATTTGGGACTCTGCCATATT170.24886546625677058No Hit
AACTCCTGTTTAGGCTTTGCGGCCACTGATAGGAGGCTAAAGACCAAGTC160.23422632118284292No Hit
GCAAAAGTTCTCAGGGATGTCCGAGATGTGTAAGCGCAGCGGCTAAAGAC130.19030888596105988No Hit
GCAAAAGTTCTCAGGGATGTCGCGGTGAGTTCAGGCTTTTTCATGGTTTA130.19030888596105988No Hit
CGCCAGGAGGAATCATTCAGGCCTTGCAGAATTTGGGACTCTGCCATATT110.1610305958132045No Hit
CGCCAGGCAGGATCATTCAGGCCTTGCAGAATTTGGGACTCTGCCATATT100.1463914507392768No Hit
GCAAAAGTTCTCAGGGATGTCCAGGCTTTTTCATGGTTTAGTTCCTCACA100.1463914507392768No Hit
CGCCAGGCAGGAATCATTCAGGCCTTGCAGAATTTGGGACTCTGCCATAC90.13175230566534915No Hit
AAGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACA90.13175230566534915No Hit
GCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC80.11711316059142146No Hit
GCAAAAGTTCTCAGGGATGTTAGAGATGTGTAAGCGCAGCGGCTAAAGAC80.11711316059142146No Hit
AGCAACAATGATCAGCAGGCTAAAGACCAAGTCTCTGCTACCGTAGGTCT80.11711316059142146No Hit
CGCCAGGCAGGAATCACTCAGGCCTTGCAGAATTTGGGACTCTGCCATAT80.11711316059142146No Hit
CGCCAGGCAGAATCATTCAGGCCTTGCAGAATTTGGGACTCTGCCATATT70.10247401551749377No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATCTTCAAACTCACAC70.10247401551749377No Hit
CGCCAGGCAGGAATCATCCAGGCCTTGCAGAATTTGGGACTCTGCCATAT70.10247401551749377No Hit
GCAAAGTTCTCAGGGATGTGTAAGCGCAGCGGCTAAAGACCAAGTCTCTG70.10247401551749377No Hit
CGCCAGGCAGGAATCATTCAGGCCTTGTAGAATTTGGGACTCTGCCATAT70.10247401551749377No Hit
CGCCAGGCAGGAATCATTCAGGCCTTGCAGAATTTGGAACTCTGCCATAT70.10247401551749377No Hit
CGCCAGGCAGGAATCATTCAGGCCTTGCAGAATTTGGGACTCCGCCATAT70.10247401551749377No Hit
CGCCAGGCAGGAATCATTCAGGCCTTGCGGAATTTGGGACTCTGCCATAT70.10247401551749377No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)