FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008145067

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008145067
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34944
Total Bases5 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGCTTACACATCTCTTCCACTTCTTTTACTCTGGCATATTTGAGAC1575445.08356227106227No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC601517.213255494505493No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT32749.369276556776557No Hit
TGTTGATTCAAGTGCTTTGGACTGTAGAACCATGTCGTCAGTGTAGATCT15764.51007326007326No Hit
TGCTGATCATTGTTGCTTTGGACTGTAGAACCATGTCGTCAGTGTAGATC10643.0448717948717947No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAAATCAAGTCAAATTT6201.7742673992673992No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAATCAAGTCAAATTTC5571.5939789377289375No Hit
TCCTATCAGTGGCCGCAAGGCCTAAACAGGAGTTTTGGACTGTAGAACCA3410.9758470695970696No Hit
GCTGCGCTTACAATCTCTTCCACTTCTTTTACTCTGGCATATTTGAGACT3300.9443681318681318No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAATCAAGTCAAATTTCT1940.5551739926739927No Hit
GTTGTTTAGAATGAGGAGAGAGGCCCTGGGCTTCACTTACTCTTCATTCA1860.5322802197802198No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAAAATCAAGTCAAATT1750.500801282051282No Hit
GTGCGCTTACACATCTCTTCCACTTCTTTTACTCTGGCATATTTGAGACT1500.4292582417582418No Hit
GTTGCGCTTACACATCTCTTCCACTTCTTTTACTCTGGCATATTTGAGAC1040.2976190476190476No Hit
GCTGCGTTTACACATCTCTTCCACTTCTTTTACTCTGGCATATTTGAGAC990.28331043956043955No Hit
GCTGCGCTTACATATCTCTTCCACTTCTTTTACTCTGGCATATTTGAGAC900.25755494505494503No Hit
GCTGCGCTTACACATCTCTTCTAAGCAGTGGTATCAACGCAGAGTACATG900.25755494505494503Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCTATCAGTGGCCGCAAAGCCTAAACAGGAGTTTTGGACTGTAGAACCA830.23752289377289376No Hit
GCGCGCTTACACATCTCTTCCACTTCTTTTACTCTGGCATATTTGAGACT790.22607600732600736No Hit
TGCTGATCATTGTTGCTTTGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT750.2146291208791209No Hit
GCTGCGCTTACACACCTCTTCCACTTCTTTTACTCTGGCATATTTGAGAC560.16025641025641024No Hit
GCTGCGTTACACATCTCTTCCACTTCTTTTACTCTGGCATATTTGAGACT530.15167124542124544No Hit
TGCTGTCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT530.15167124542124544No Hit
GCTGCGCTTACACATCTCTGCTAAGCAGTGGTATCAACGCAGAGTACATG510.1459478021978022Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTGTGCTTACACATCTCTTCCACTTCTTTTACTCTGGCATATTTGAGAC400.11446886446886448No Hit
GCTGCGCTTACACATCTCTTCCACTTCTTTTACTCTGACATATTTGAGAC400.11446886446886448No Hit
GCTGCGCTTACACATCTCTTCCACTTCCTTTACTCTGGCATATTTGAGAC390.11160714285714285No Hit
TGCTGATCATTGTTGTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT370.10588369963369963No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAATCAAGTCAAATTTCTT350.1001602564102564No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)