FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008145075

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008145075
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21297
Total Bases3 Mbp
Sequences flagged as poor quality0
Sequence length139-145
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT522924.552753909001268No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC503323.632436493402828No Hit
TGTTGATTCAAGTGCTTTGGACTGTAGAACCATGTCGTCAGTGTAGATCT14826.958726581208621No Hit
GCTGCGCTTACACATCTCTTCCACTTCTTTTACTCTGGCATATTTGAGAC14506.80847067662112No Hit
CAACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACA14326.7239517302906515No Hit
TGCTGATCATTGTTGCTTTGGACTGTAGAACCATGTCGTCAGTGTAGATC11325.315302624782833No Hit
GCTGCGCTTACACATCTCTGAGAACTTTTGCTTGGACTGTAGAACCATGT5242.460440437620322No Hit
GCTGCGCTTACACATCCCTGAGAACTTTTGCTTGGACTGTAGAACCATGT4241.9908907357843828No Hit
TCCTATCAGTGGCCGCAAGGCCTAAACAGGAGTTTTGGACTGTAGAACCA3891.8265483401418041No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAAATCAAGTCAAATTT3051.432126590599615No Hit
GTTGTTTAGAATGAGGAGAGAGGCCCTGGGCTTCACTTACTCTTCATTCA2541.1926562426632858No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAATCAAGTCAAATTTC1770.8311029722496126No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAAAATCAAGTCAAATT1370.6432830915152369No Hit
TCCTATCAGTGGCCGCAAAGCCTAAACAGGAGTTTTGGACTGTAGAACCA920.43198572568906424No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCTTC890.417899234633986No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAATCAAGTCAAATTTCT760.3568577733953139No Hit
GCTGCGCTTACAATCTCTTCCACTTCTTTTACTCTGGCATATTTGAGACT480.2253838568812509No Hit
GCTGCGCTTACACATCTCTGACATCCCTGAGAACTTTTGCTTGGACTGTA470.22068835986289148No Hit
TCCTATCAGTGGCCGCAAGGAGGGGAGAGGCCCTGGGCTTCACTTACTCT450.2112973658261727No Hit
TGCTGATCATTGTTGCTTTGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT440.20660186880781328No Hit
TCCTATCAGTGGCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT370.17373338967929755No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAAAAATCAAGTCAAAT320.1502559045875006No Hit
TGCTGTCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT320.1502559045875006No Hit
TCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT290.1361694135324224No Hit
GCTGCGCTTACACATCTCGGACATCCCTGAGAACTTTTGCTTGGACTGTA280.131473916514063No Hit
TGCTGATCATTGTTGCTTTGGAGGTGAGGAAAAGTCTGGAGTATTTCCTC260.12208292247734423No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTCCACTTACTCT240.11269192844062545No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAGGTCTGGAGTATTTCCTC240.11269192844062545No Hit
CAACCAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG220.10330093440390664No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)