FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008145138

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008145138
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36964
Total Bases5.3 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCCAGGCAGGAATCATTCAGGCCTTGCAGAATTTGGGACTCTGCCATAT1741047.0998809652635No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACAC582615.761281246618331No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA30048.126826101071313No Hit
AACTCCTGTTTAGGCCTTGCAGAATTTGGGACTCTGCCATATTCTTTCCG19045.150957688561844No Hit
AGCACTTGAATCAACAGGCTAAAGACCAAGTCTCTGCTACCGTACATTCT14773.995779677524078No Hit
AGCAACAATGATCAGCAGGCTAAAGACCAAGTCTCTGCTACCGTACATTC10362.802726977599827No Hit
GCAAAAGTTCTCAGAGATGTGTAAGCGCAGCGGCTAAAGACCAAGTCTCT5951.6096742776755761No Hit
GCAAAAGTTCTCAGGGATGTGTAAGCGCAGCGGCTAAAGACCAAGTCTCT5621.5203982253002921No Hit
AACTCCTGTTTAGGCCTTGCGGCCACTGATAGGAGGCTAAAGACCAAGTC3821.0334379396169247No Hit
CGCAGGCAGGAATCATTCAGGCCTTGCAGAATTTGGGACTCTGCCATATT2170.5870576777405043No Hit
GCCAGGCAGGAATCATTCAGGCCTTGCAGAATTTGGGACTCTGCCATATT1410.38145222378530463No Hit
AGCACTTGAATAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC1340.36251487934206256No Hit
CGCCAGGAGGAATCATTCAGGCCTTGCAGAATTTGGGACTCTGCCATATT1130.3057028460123363No Hit
CGCCGGCAGGAATCATTCAGGCCTTGCAGAATTTGGGACTCTGCCATATT810.21913212855751543No Hit
CGCCAGCAGGAATCATTCAGGCCTTGCAGAATTTGGGACTCTGCCATATT780.21101612379612597No Hit
AACTCCTGTTTAGGCTTTGCGGCCACTGATAGGAGGCTAAAGACCAAGTC750.20290011903473648No Hit
CGCCAGGCAGAATCATTCAGGCCTTGCAGAATTTGGGACTCTGCCATATT570.15420409046639974No Hit
CGCCAGGCGGAATCATTCAGGCCTTGCAGAATTTGGGACTCTGCCATATT520.14067741586408397No Hit
GCAAAAGTTCTCAGGGATGTCAGAGATGTGTAAGCGCAGCGGCTAAAGAC450.1217400714208419No Hit
CGCCAGGCAGGAATCATTCAGGCCTTGCAGAATTTGGGACTCTGTCATAT440.11903473650037874No Hit
CGCCAGGCAGGAATCATTCGGGCCTTGCAGAATTTGGGACTCTGCCATAT440.11903473650037874No Hit
CGCCAGGCAGGAATCATTCAGGCCTTGCGGAATTTGGGACTCTGCCATAT430.11632940157991559No Hit
ACTCCTGTTTAGGCCTTGCAGAATTTGGGACTCTGCCATATTCTTTCCGT410.11091873173898928No Hit
AGCACTTGAATCAACAGGCTACAGAGACTGAGCCATTTTCAAACTCACAC390.10550806189806299No Hit
CCCAGGCAGGAATCATTCAGGCCTTGCAGAATTTGGGACTCTGCCATATT390.10550806189806299No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)