FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008145147

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008145147
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5118
Total Bases742 kbp
Sequences flagged as poor quality0
Sequence length143-145
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC181935.54122704181321No Hit
TGTTGATTCAAGTGCTTTGGACTGTAGAACCATGTCGTCAGTGTAGATCT93518.268855021492772No Hit
TGCTGATCATTGTTGCTTTGGACTGTAGAACCATGTCGTCAGTGTAGATC66112.915201250488472No Hit
TCCTATCAGTGGCCGCAAGGCCTAAACAGGAGTTTTGGACTGTAGAACCA2945.74443141852286No Hit
GCTGCGCTTACACATCCCTGAGAACTTTTGCTTGGACTGTAGAACCATGT2755.373192653380227No Hit
GCTGCGCTTACACATCTCTGAGAACTTTTGCTTGGACTGTAGAACCATGT2474.82610394685424No Hit
TCCTATCAGTGGCCGCAAAGCCTAAACAGGAGTTTTGGACTGTAGAACCA941.836654943337241No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAAATCAAGTCAAATTT250.48847205939820243No Hit
TGCTGATCATTGTTGCTTTGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT220.4298554122704181No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAATCAAGTCAAATTTC160.31262211801484957No Hit
TGTTATTCAAGTGCTTTGGACTGTAGAACCATGTCGTCAGTGTAGATCTC150.29308323563892147No Hit
TGCTGATCATTGTTGCTTTGGAGGTGAGGAAAAGTCTGGAGTATTTCCTC150.29308323563892147No Hit
GCTGCGCTTACACATCTCGGACATCCCTGAGAACTTTTGCTTGGACTGTA130.25400547088706527No Hit
TGCTGATCATTGTTGCTTTGGGGGTGGGGAAAAGTCTGGAGTATTTCCTC120.23446658851113714No Hit
GCTGCGCTTACACATCTCTGACATCCCTGAGAACTTTTGCTTGGACTGTA110.21492770613520906No Hit
TGCTGTCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT100.19538882375928096No Hit
TGCTGATCATTGTTGTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT90.1758499413833529No Hit
TGTTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC90.1758499413833529No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTCCCTC90.1758499413833529No Hit
GCTGCGCTTACACATCCCTGAGAACTTTGCTTGGACTGTAGAACCATGTC80.15631105900742479No Hit
TGCTGATCATTGTTGCTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT80.15631105900742479No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAAAATCAAGTCAAATT80.15631105900742479No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGGAAAGTCTGGAGTATTTCCTC80.15631105900742479No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAGGTCTGGAGTATTTCCTC70.13677217663149666No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCCC70.13677217663149666No Hit
TGTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT60.11723329425556857No Hit
TGTTGATCATTGTTGCTTTGGACTGTAGAACCATGTCGTCAGTGTAGATC60.11723329425556857No Hit
GCTGCGCTTACACATCTCTAACATCCCTGAGAACTTTTGCTTGGACTGTA60.11723329425556857No Hit
GCTGCGCTTACACATCTCTTCTAAGCAGTGGTATCAACGCAGAGTACATG60.11723329425556857Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)