FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008145150

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008145150
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26857
Total Bases3.8 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA631423.509699519678296No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACAC613522.843206612801133No Hit
CGCCAGGCAGGAATCATTCAGGCCTTGCAGAATTTGGGACTCTGCCATAT338312.596343597572327No Hit
AACTCCTGTTTAGGCCTTGCAGAATTTGGGACTCTGCCATATTCTTTCCG16736.2292884536619875No Hit
AGCACTTGAATCAACAGGCTAAAGACCAAGTCTCTGCTACCGTAGGCTGT16606.180883940872025No Hit
AGCAACAATGATCAGCAGGCTAAAGACCAAGTCTCTGCTACCGTAGGCTG12324.587258442864058No Hit
CGCCAGGCAGGAATCATTCAGGATTGGATTATGAAAATAAAGACTGGTGT10053.7420411810701117No Hit
GCAAAAGTTCTCAGGGATGTGTAAGCGCAGCGGCTAAAGACCAAGTCTCT7812.907994191458465No Hit
GCAAAAGTTCTCAGAGATGTGTAAGCGCAGCGGCTAAAGACCAAGTCTCT7462.777674349331645No Hit
AACTCCTGTTTAGGCCTTGCGGCCACTGATAGGAGGCTAAAGACCAAGTC3861.4372416874557843No Hit
AGCACTTGAATAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC1560.5808541534795397No Hit
AACTCCTGTTTAGGCTTTGCGGCCACTGATAGGAGGCTAAAGACCAAGTC810.30159734892206874No Hit
GGTGTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG730.2718099564359385No Hit
GGTGTTTGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG620.23085229176750938No Hit
GCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC490.18244777897754777No Hit
GGTGTTTGGGATGGATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG370.1377666902483524No Hit
CGCAGGCAGGAATCATTCAGGCCTTGCAGAATTTGGGACTCTGCCATATT330.12287299400528726No Hit
AGCACTTGAATCACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC330.12287299400528726No Hit
ACTCCTGTTTAGGCCTTGCAGAATTTGGGACTCTGCCATATTCTTTCCGT310.11542614588375469No Hit
GCCAGGCAGGAATCATTCAGGCCTTGCAGAATTTGGGACTCTGCCATATT310.11542614588375469No Hit
AGCACTTGAATCAACAGGCTACAGAGACTGAGCCATTTTCAAACTCACAC300.11170272182298843No Hit
GTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG300.11170272182298843No Hit
CGCCAGGAGGAATCATTCAGGCCTTGCAGAATTTGGGACTCTGCCATATT300.11170272182298843No Hit
GCAAAAGTTCTCAGGGATGTCGAGCAACTCTGCGTTGATACCACTGCTTC300.11170272182298843Clontech SMART CDS Primer II A (95% over 24bp)
GGTGTTTGGGTGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG290.10797929776222215No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACCCACAC290.10797929776222215No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)