FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008145154

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008145154
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24436
Total Bases3.5 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACAC643926.350466524799476No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA479219.610410869209364No Hit
CGCCAGGCAGGAATCATTCAGGCCTTGCAGAATTTGGGACTCTGCCATAT281511.519888688819774No Hit
AACTCCTGTTTAGGCCTTGCAGAATTTGGGACTCTGCCATATTCTTTCCG253910.390407595351121No Hit
AGCACTTGAATCAACAGGCTAAAGACCAAGTCTCTGCTACCGTATGGTGC17997.362088721558356No Hit
AGCAACAATGATCAGCAGGCTAAAGACCAAGTCTCTGCTACCGTATGGTG13945.704697986577181No Hit
GCAAAAGTTCTCAGAGATGTGTAAGCGCAGCGGCTAAAGACCAAGTCTCT5042.062530692421018No Hit
GCAAAAGTTCTCAGGGATGTGTAAGCGCAGCGGCTAAAGACCAAGTCTCT4281.7515141594368966No Hit
AACTCCTGTTTAGGCCTTGCGGCCACTGATAGGAGGCTAAAGACCAAGTC4041.6532984121787524No Hit
AGCACTTGAATAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC1300.5320019643149452No Hit
AACTCCTGTTTAGGCTTTGCGGCCACTGATAGGAGGCTAAAGACCAAGTC820.33557046979865773No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGTA580.237354722540514No Hit
GCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC540.22098543133082338No Hit
GGTGTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG520.21280078572597808No Hit
GCAAAAGTTCTCAGGGATGTCAGAGATGTGTAAGCGCAGCGGCTAAAGAC510.2087084629235554No Hit
GGTGTTTGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG420.17187755770175153No Hit
ACTCCTGTTTAGGCCTTGCAGAATTTGGGACTCTGCCATATTCTTTCCGT410.16778523489932887No Hit
CGCAGGCAGGAATCATTCAGGCCTTGCAGAATTTGGGACTCTGCCATATT380.1555082664920609No Hit
AAGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACA370.15141594368963823No Hit
AACTCCTGTTAGGCCTTGCAGAATTTGGGACTCTGCCATATTCTTTCCGT360.1473236208872156No Hit
GCCAGGCAGGAATCATTCAGGCCTTGCAGAATTTGGGACTCTGCCATATT340.13913897528237026No Hit
AGCACTTGAATAACAGGCTAAAGACCAAGTCTCTGCTACCGTATGGTGCT300.12276968407267964No Hit
GGTGTTTGGGTGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG260.10640039286298904No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)