FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008145203

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008145203
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26524
Total Bases3.8 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGCTTACACATCTCTTCCACTTCTTTTACTCTGGCATATTTGAGAC930935.09651636253959No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT382114.405821143115668No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC365513.779972854773037No Hit
TGTTGATTCAAGTGCTTTGGACTGTAGAACCATGTCGTCAGTGTAGATCT14385.421505052028352No Hit
TGCTGATCATTGTTGCTTTGGACTGTAGAACCATGTCGTCAGTGTAGATC10674.022771829286683No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAAATCAAGTCAAATTT6682.5184738350173426No Hit
GCTGCGCTTACACATCCCTGAGAACTTTTGCTTGGACTGTAGAACCATGT5492.0698235560247324No Hit
GCTGCGCTTACACATCTCTGAGAACTTTTGCTTGGACTGTAGAACCATGT4931.8586940129693863No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAATCAAGTCAAATTTC4051.5269190167395565No Hit
TCCTATCAGTGGCCGCAAGGCCTAAACAGGAGTTTTGGACTGTAGAACCA3021.138591464334188No Hit
GTTGTTTAGAATGAGGAGAGAGGCCCTGGGCTTCACTTACTCTTCATTCA1890.7125622078117931No Hit
GCTGCGCTTACAATCTCTTCCACTTCTTTTACTCTGGCATATTTGAGACT1880.7087920374000906No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAAAATCAAGTCAAATT1870.7050218669883879No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAATCAAGTCAAATTTCT1430.5391343688734731No Hit
CAACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACA1330.501432664756447No Hit
GTGCGCTTACACATCTCTTCCACTTCTTTTACTCTGGCATATTTGAGACT850.32046448499472174No Hit
GCTGCGCTTACACATCTCTGACATCCCTGAGAACTTTTGCTTGGACTGTA850.32046448499472174No Hit
TCCTATCAGTGGCCGCAAAGCCTAAACAGGAGTTTTGGACTGTAGAACCA830.31292414417131653No Hit
GTTGCGCTTACACATCTCTTCCACTTCTTTTACTCTGGCATATTTGAGAC750.28276278087769563No Hit
GCTGCGTTTACACATCTCTTCCACTTCTTTTACTCTGGCATATTTGAGAC580.21866988387875133No Hit
GCTGCGCTTACACATCTCTTCTACTTCTTTTACTCTGGCATATTTGAGAC560.2111295430553461No Hit
TGCTGATCATTGTTGCTTTGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT550.20735937264364349No Hit
GCTGCGCTTACATATCTCTTCCACTTCTTTTACTCTGGCATATTTGAGAC520.19604886140853567No Hit
TCCTATCAGTGGCCGCAAGGAGGGGAGAGGCCCTGGGCTTCACTTACTCT470.17719800935002264No Hit
GCGCGCTTACACATCTCTTCCACTTCTTTTACTCTGGCATATTTGAGACT450.1696576685266174No Hit
GCTGCGCTTACACATCTCTTCTAAGCAGTGGTATCAACGCAGAGTACATG390.14703664605640177Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTGCGTTACACATCTCTTCCACTTCTTTTACTCTGGCATATTTGAGACT380.14326647564469913No Hit
GCTGCGCTTACACACCTCTTCCACTTCTTTTACTCTGGCATATTTGAGAC380.14326647564469913No Hit
GCTGCGCTTACACATCTCTTCCACTTCTTTTACTCTGGCATATTTGAGGC370.13949630523299653No Hit
GCTGCGCTTACACATCTTTTCCACTTCTTTTACTCTGGCATATTTGAGAC360.13572613482129392No Hit
TCCTATCAGTGGCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT360.13572613482129392No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAAAAATCAAGTCAAAT350.13195596440959131No Hit
GCTGCGCTTACACATCTCTTCCACTTCCTTTACTCTGGCATATTTGAGAC320.12064545317448348No Hit
GCTGCGCTTACACATCTCGGACATCCCTGAGAACTTTTGCTTGGACTGTA310.11687528276278089No Hit
GCTGCGCTTACACATCTCTTCCACTTCTTTTACTCTGGCATATTTGGGAC310.11687528276278089No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)