FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008145252

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008145252
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23440
Total Bases3.3 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACAC567424.206484641638227No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA531522.67491467576792No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAATTCACAC18067.704778156996587No Hit
AGCACTTGAATCAACAGGCTAAAGACCAAGTCTCTGCTACCGTAACCATG17797.589590443686006No Hit
AGCAACAATGATCAGCAGGCTAAAGACCAAGTCTCTGCTACCGTAACCAT12675.4052901023890785No Hit
CGCCAGGCAGGAATCATTCAGGCCTTGCAGAATTTGGGACTCTGCCATAT11324.829351535836177No Hit
AACTCCTGTTTAGGCCTTGCAGAATTTGGGACTCTGCCATATTCTTTCCG10564.505119453924915No Hit
GCAAAAGTTCTCAGAGATGTGTAAGCGCAGCGGCTAAAGACCAAGTCTCT6962.9692832764505117No Hit
GCAAAAGTTCTCAGGGATGTGTAAGCGCAGCGGCTAAAGACCAAGTCTCT4952.111774744027304No Hit
AACTCCTGTTTAGGCCTTGCGGCCACTGATAGGAGGCTAAAGACCAAGTC4451.8984641638225257No Hit
GCAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGC3351.4291808873720135No Hit
AACTCCTGTTTAGGCTTTGCGGCCACTGATAGGAGGCTAAAGACCAAGTC1230.5247440273037542No Hit
AGCACTTGAATAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC1090.46501706484641636No Hit
GCAAAAGTTCTCAGGGATGTCAGAGATGTGTAAGCGCAGCGGCTAAAGAC970.41382252559726956No Hit
GGTGTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG610.26023890784982934No Hit
GCAAAAGTTCTCAGGGATGTCCGAGATGTGTAAGCGCAGCGGCTAAAGAC590.2517064846416382No Hit
GCAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTG580.24744027303754265No Hit
GGTGTTTGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG410.17491467576791808No Hit
GCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC390.16638225255972697No Hit
GTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG370.15784982935153583No Hit
AAGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACA360.15358361774744025No Hit
AGCACTTGAATAACAGGCTTCAGAGACTGAGCCATTTTCAAATTCACACC300.12798634812286688No Hit
AGCACTTGAATAACAGGCTAAAGACCAAGTCTCTGCTACCGTAACCATGA290.12372013651877133No Hit
GGTGTTTGGGATGGATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG280.11945392491467575No Hit
GCAAAAGTTCTCAGGGATGTGTAAGCGCAGCCTTCCCATGTACTCTGCGT280.11945392491467575No Hit
GGTGTTTGGGTGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG260.11092150170648465No Hit
AGCACTTGAATAAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACAC250.10665529010238908No Hit
AGCACTTGAATCACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC250.10665529010238908No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)