FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008145294

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008145294
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10493
Total Bases1.5 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA274026.112646526255602No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACAC242023.06299437720385No Hit
AGCACTTGAATCAACAGGCTAAAGACCAAGTCTCTGCTACCGTATGAGAA9949.472981987991995No Hit
AACTCCTGTTTAGGCCTTGCAGAATTTGGGACTCTGCCATATTCTTTCCG6916.58534260935862No Hit
AGCAACAATGATCAGCAGGCTAAAGACCAAGTCTCTGCTACCGTATGAGA6876.5472219574954735No Hit
GCAAAAGTTCTCAGAGATGTGTAAGCGCAGCGGCTAAAGACCAAGTCTCT5265.012865720003812No Hit
GCAAAAGTTCTCAGGGATGTGTAAGCGCAGCGGCTAAAGACCAAGTCTCT4594.374344801296102No Hit
AACTCCTGTTTAGGCCTTGCGGCCACTGATAGGAGGCTAAAGACCAAGTC1591.5152959115600877No Hit
GCAAAAGTTCTCAGGGATGTCGCAACTCTGCGTTGATACCACCCCATGTA810.7719432002287239No Hit
AGCACTTGAATAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC610.5813399409129896No Hit
AACTCCTGTTTAGGCTTTGCGGCCACTGATAGGAGGCTAAAGACCAAGTC430.4097970075288287No Hit
GGTGTTTGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG280.26684456304202797No Hit
GCAAAAGTTCTCAGGGATGTCAGAGATGTGTAAGCGCAGCGGCTAAAGAC260.2477842371104546No Hit
GCAAAAGTTCTCAGGGATGTAGCAACTCTGCGTTGATACCACCCCATGTA250.23825407414466787No Hit
GGTGTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG230.21919374821309442No Hit
GCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC220.20966358524730772No Hit
GCAAAAGTTCTCAGGGATGTCCGAGATGTGTAAGCGCAGCGGCTAAAGAC220.20966358524730772No Hit
GCAAAAGTTCTCAGGGATGTCGCAACTCTGCGTTGATACCCCATGTACTC210.20013342228152103No Hit
GGTGTTTGGGTGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG190.1810730963499476No Hit
AGCACTTGAATCACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC190.1810730963499476No Hit
AGCACTTGAATCAACAGGCTAAAGACCAAGTCTCTGCTACCGAATGAGAA190.1810730963499476No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTCCGCAGAAGCAGCA170.16201277041837414No Hit
AAGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACA160.15248260745258743No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACACACAC150.14295244448680072No Hit
ACTCCTGTTTAGGCCTTGCAGAATTTGGGACTCTGCCATATTCTTTCCGT140.13342228152101399No Hit
GGTGTTTGGGATGGATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG140.13342228152101399No Hit
GGTGTTTGGGATGGAATGGTGGTCCACGCAGGTGGTTCGCAGAAGCAGCA130.1238921185552273No Hit
GCAAAAGTTCTCAGGGATGTCGCAACTCTGCGTTGATACCACTGCTTCCC130.1238921185552273Clontech Universal Primer Mix Long (96% over 26bp)
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCACAGAAGCAGCA130.1238921185552273No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCGGCA120.11436195558944058No Hit
AACTCCTGTTAGGCCTTGCAGAATTTGGGACTCTGCCATATTCTTTCCGT120.11436195558944058No Hit
AGCACTTGAATCAACAGGCTACAGAGACTGAGCCATTTTCAAACTCACAC120.11436195558944058No Hit
GCAAAAGTTCTCAGGGATGTCGCAACTCTGCGTTGATACCACCCATGTAC120.11436195558944058No Hit
AGCACTTGAATAACAGGCTAAAGACCAAGTCTCTGCTACCGTATGAGAAG120.11436195558944058No Hit
GGTGTTTGGGATGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG110.10483179262365386No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)