FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008145306

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008145306
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22520
Total Bases3.2 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACAC649928.85879218472469No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA443619.69804618117229No Hit
AACTCCTGTTTAGGCCTTGCAGAATTTGGGACTCTGCCATATTCTTTCCG22019.77353463587922No Hit
CGCCAGGCAGGAATCATTCAGGCCTTGCAGAATTTGGGACTCTGCCATAT19018.441385435168739No Hit
AGCACTTGAATCAACAGGCTAAAGACCAAGTCTCTGCTACCGTATGACTA17427.735346358792185No Hit
AGCAACAATGATCAGCAGGCTAAAGACCAAGTCTCTGCTACCGTATGACT12715.643872113676732No Hit
AACTCCTGTTTAGGCCTTGCGGCCACTGATAGGAGGCTAAAGACCAAGTC4712.091474245115453No Hit
GCAAAAGTTCTCAGAGATGTGTAAGCGCAGCGGCTAAAGACCAAGTCTCT4301.9094138543516874No Hit
GCAAAAGTTCTCAGGGATGTGTAAGCGCAGCGGCTAAAGACCAAGTCTCT3891.7273534635879217No Hit
AGCACTTGAATAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC1550.6882770870337478No Hit
AACTCCTGTTTAGGCTTTGCGGCCACTGATAGGAGGCTAAAGACCAAGTC1150.5106571936056838No Hit
GGTGTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG470.20870337477797513No Hit
AGCACTTGAATCACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC390.17317939609236233No Hit
AAGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACA370.16429840142095914No Hit
GGTGTTTGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG370.16429840142095914No Hit
GCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC340.15097690941385436No Hit
AGCACTTGAATAACAGGCTAAAGACCAAGTCTCTGCTACCGTATGACTAG340.15097690941385436No Hit
AGCACTTGAATCAACAGGCTACAGAGACTGAGCCATTTTCAAACTCACAC330.14653641207815277No Hit
GGTGTTTGGGATGGATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG310.13765541740674955No Hit
ACTCCTGTTTAGGCCTTGCAGAATTTGGGACTCTGCCATATTCTTTCCGT280.12433392539964476No Hit
CGCAGGCAGGAATCATTCAGGCCTTGCAGAATTTGGGACTCTGCCATATT280.12433392539964476No Hit
AGCACTTGAATAAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACAC280.12433392539964476No Hit
AACTCCTGTTAGGCCTTGCAGAATTTGGGACTCTGCCATATTCTTTCCGT270.11989342806394317No Hit
AGCACTTGAATCAACAGGCTAAAGACCAAGTCTCTGCTACCGAATGACTA270.11989342806394317No Hit
GGTGTTTGGGTGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG260.11545293072824157No Hit
AACTCTGTTTAGGCCTTGCAGAATTTGGGACTCTGCCATATTCTTTCCGT260.11545293072824157No Hit
GTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG250.11101243339253995No Hit
GGTGTTTGGGATGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG250.11101243339253995No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGGGCCATTTTCAAACTCACAC240.10657193605683836No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)