FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008145366

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008145366
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20291
Total Bases2.9 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACAC620930.599773298506726No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA561327.66251047262333No Hit
AACTCCTGTTTAGGCCTTGCAGAATTTGGGACTCTGCCATATTCTTTCCG17488.614656744369425No Hit
AGCACTTGAATCAACAGGCTAAAGACCAAGTCTCTGCTACCGTATGGATG15877.821201517914346No Hit
AGCAACAATGATCAGCAGGCTAAAGACCAAGTCTCTGCTACCGTATGGAT12266.042087625055443No Hit
AACTCCTGTTTAGGCCTTGCGGCCACTGATAGGAGGCTAAAGACCAAGTC4482.2078754127445666No Hit
GCAAAAGTTCTCAGAGATGTGTAAGCGCAGCGGCTAAAGACCAAGTCTCT3141.5474841062540041No Hit
GCAAAAGTTCTCAGGGATGTGTAAGCGCAGCGGCTAAAGACCAAGTCTCT2991.4735597062737174No Hit
AGCACTTGAATAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC1480.72938741313883No Hit
AACTCCTGTTTAGGCTTTGCGGCCACTGATAGGAGGCTAAAGACCAAGTC1160.5716820265142181No Hit
GGTGTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG610.3006258932531664No Hit
GGTGTTTGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG560.2759844265930708No Hit
AGCACTTGAATCACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC360.17741855995268838No Hit
GCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC340.16756197328865013No Hit
AGCACTTGAATAACAGGCTAAAGACCAAGTCTCTGCTACCGTATGGATGA300.14784879996057365No Hit
GGTGTTTGGGTGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG300.14784879996057365No Hit
AAGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACA290.14292050662855452No Hit
GGTGTTTGGGATGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG270.13306391996451628No Hit
AGCACTTGAATAAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACAC260.12813562663249717No Hit
ACTCCTGTTTAGGCCTTGCAGAATTTGGGACTCTGCCATATTCTTTCCGT250.12320733330047805No Hit
AGCACTTGAATCAACAGGCTACAGAGACTGAGCCATTTTCAAACTCACAC240.11827903996845893No Hit
GCAAAAGTTCTCAGGGATGTGTAAGCGCAGCCTTCCCATGTACTCTGCGT230.1133507466364398No Hit
GCAAAAGTTCTCAGGGATGTCAGAGATGTGTAAGCGCAGCGGCTAAAGAC220.10842245330442069No Hit
GGTGTTTGGGATGGATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG210.10349415997240156No Hit
GTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG210.10349415997240156No Hit
AGCACTTGAATCAACAGGCTAAAGACCAAGTCTCTGCTACCGAATGGATG210.10349415997240156No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)