FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008145372

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008145372
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21678
Total Bases3.1 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA726733.52246517206384No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACAC635429.310822031552725No Hit
AGCACTTGAATCAACAGGCTAAAGACCAAGTCTCTGCTACCGTAAGGAGA16267.500691945751453No Hit
AGCAACAATGATCAGCAGGCTAAAGACCAAGTCTCTGCTACCGTAAGGAG12995.992250207583726No Hit
GCAAAAGTTCTCAGAGATGTGTAAGCGCAGCGGCTAAAGACCAAGTCTCT5242.4171971584094476No Hit
GCAAAAGTTCTCAGGGATGTGTAAGCGCAGCGGCTAAAGACCAAGTCTCT5042.324937724882369No Hit
CGCCAGGCAGGAATCATTCAGGATTGGATTATGAAAATAAAGACTGGTGT4572.1081280560937357No Hit
AACTCCTGTTTAGGCCTTGCGGCCACTGATAGGAGGCTAAAGACCAAGTC3301.5222806531967894No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGGAGCAGCA2130.9825629670633822No Hit
AGCACTTGAATAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC1700.7842051849801641No Hit
AACTCCTGTTTAGGCCTTGCAGAATTTGGGACTCTGCCATATTCTTTCCG920.4243933942245595No Hit
AACTCCTGTTTAGGCTTTGCGGCCACTGATAGGAGGCTAAAGACCAAGTC740.3413599040501891No Hit
GGTGTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG690.3182950456684196No Hit
GGTGTTTGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG610.2813912722575883No Hit
AAGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACA510.23526155549404926No Hit
GCAAAAGTTCTCAGGGATGTGTAAGCGCAGCCTTCCCATGTACTCTGCGT450.20758372543592582No Hit
GGTGTTTGGGATGGATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG400.1845188670541563No Hit
GGTGTTTGGGTGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG400.1845188670541563No Hit
GTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG400.1845188670541563No Hit
AGCACTTGAATAACAGGCTAAAGACCAAGTCTCTGCTACCGTAAGGAGAA330.15222806531967895No Hit
GCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC300.1383891502906172No Hit
GCAAAAGTTCTCAGGGATGTGCTCGAAGCAACTCTGCGTTGATACCACCC280.12916320693790942No Hit
AGCACTTGAATCACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC280.12916320693790942No Hit
GGTTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG270.1245502352615555No Hit
AGCACTTGAATAAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACAC260.11993726358520157No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCGGCA240.11071132023249376No Hit
AGCACTTGAATCAACAGGTTTCAGAGACTGAGCCATTTTCAAACTCACAC240.11071132023249376No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGGGCCATTTTCAAACTCACAC220.10148537687978595No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)