FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008145382

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008145382
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21693
Total Bases3.1 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACAC541224.948139952980224No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA458521.135850274282024No Hit
CGCCAGGCAGGAATCATTCAGGCCTTGCAGAATTTGGGACTCTGCCATAT296913.68644263126354No Hit
AGCACTTGAATCAACAGGCTAAAGACCAAGTCTCTGCTACCGTAGTCTTG14506.684183838104457No Hit
AGCAACAATGATCAGCAGGCTAAAGACCAAGTCTCTGCTACCGTAGTCTT11395.250541649379984No Hit
AACTCCTGTTTAGGCCTTGCAGAATTTGGGACTCTGCCATATTCTTTCCG8463.8998755358871526No Hit
GCAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGC8173.7661918591250636No Hit
GCAAAAGTTCTCAGAGATGTGTAAGCGCAGCGGCTAAAGACCAAGTCTCT5702.627575715668649No Hit
GCAAAAGTTCTCAGGGATGTGTAAGCGCAGCGGCTAAAGACCAAGTCTCT4462.055962752961785No Hit
AACTCCTGTTTAGGCCTTGCGGCCACTGATAGGAGGCTAAAGACCAAGTC3821.760936707693726No Hit
AGCACTTGAATAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC1150.5301249250910433No Hit
GCAAAAGTTCTCAGGGATGTCAGAGATGTGTAAGCGCAGCGGCTAAAGAC940.4333195039874614No Hit
AACTCCTGTTTAGGCTTTGCGGCCACTGATAGGAGGCTAAAGACCAAGTC790.3641727746277601No Hit
GGTGTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG500.23048909786567096No Hit
GCAAAAGTTCTCAGGGATGTCCGAGATGTGTAAGCGCAGCGGCTAAAGAC430.19822062416447706No Hit
GGTGTTTGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG370.1705619324205965No Hit
GCAAAAGTTCTAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGCA360.1659521504632831No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCACAGAAGCAGCA340.15673258654865624No Hit
GCAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGCA320.1475130226340294No Hit
GCCAGGCAGGAATCATTCAGGCCTTGCAGAATTTGGGACTCTGCCATATT300.13829345871940257No Hit
GGTGTTTGGGATGGATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG290.13368367676208914No Hit
AAGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACA280.12907389480477574No Hit
GTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG240.11063476697552206No Hit
CGCAGGCAGGAATCATTCAGGCCTTGCAGAATTTGGGACTCTGCCATATT230.10602498501820865No Hit
GCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC220.10141520306089521No Hit
GGTGTTTGGGTGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG220.10141520306089521No Hit
AGCACTTGAATAAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACAC220.10141520306089521No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)