FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008145406

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008145406
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2703
Total Bases391.9 kbp
Sequences flagged as poor quality0
Sequence length144-145
%GC61

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACTTGAATCAACAGGCTAAAGACCAAGTCTCTGCTACCGTATTCGTT73127.044025157232703No Hit
AGCAACAATGATCAGCAGGCTAAAGACCAAGTCTCTGCTACCGTATTCGT55420.495745467998518No Hit
GCAAAAGTTCTCAGGGATGTGTAAGCGCAGCGGCTAAAGACCAAGTCTCT38414.206437291897892No Hit
GCAAAAGTTCTCAGAGATGTGTAAGCGCAGCGGCTAAAGACCAAGTCTCT28710.617832038475768No Hit
AACTCCTGTTTAGGCCTTGCGGCCACTGATAGGAGGCTAAAGACCAAGTC2248.287088420273768No Hit
AACTCCTGTTTAGGCTTTGCGGCCACTGATAGGAGGCTAAAGACCAAGTC471.7388087310395854No Hit
AGCACTTGAATAACAGGCTAAAGACCAAGTCTCTGCTACCGTATTCGTTC170.628930817610063No Hit
AGCACTTGAATCAACAGGCTAAAGACCAAGTCTCTGCTACCGAATTCGTT120.4439511653718091No Hit
CGCCAGGCAGGAATCATTCACACTGACGACATGGTTCTACAGTCCAAGCA70.25897151313355526No Hit
AACTCCTGTTTAGGCCTTGCGCCACTGATAGGAGGCTAAAGACCAAGTCT60.22197558268590456No Hit
AGCACTTGATCAACAGGCTAAAGACCAAGTCTCTGCTACCGTATTCGTTC60.22197558268590456No Hit
AGCAACAATGATCAGCAGGCTAAAGACCAAGTCTCTGCTACCGTTTGCGG60.22197558268590456No Hit
AGCAACAATGACAGCAGGCTAAAGACCAAGTCTCTGCTACCGTATTCGTT60.22197558268590456No Hit
AAGCACTTGAATCAACAGGCTAAAGACCAAGTCTCTGCTACCGTATTCGT60.22197558268590456No Hit
GCAAAAGTTCTCAGAGATGTGTAAGCGCAGCGGCTAAAGACAAAGTCTCT50.1849796522382538No Hit
GAAAAGTTCTCAGGGATGTGTAAGCGCAGCGGCTAAAGACCAAGTCTCTG50.1849796522382538No Hit
AGCACTTGAATCAACAGGCTAAAGACCAAGTCTCTGCTACCGTTGCGGCC50.1849796522382538No Hit
GCAAAAGTTCTCAGGGATGTCGATGAGGGGATCCCATGTACTCTGCGTTG50.1849796522382538No Hit
ACTCCTGTTTAGGCCTTGCGGCCACTGATAGGAGGCTAAAGACCAAGTCT50.1849796522382538No Hit
GCAAAAGTTCTCAGGGATGTCAGAGATGTGTAAGCGCAGCGGCTAAAGAC50.1849796522382538No Hit
GCAAAAGTTCTCAGGGATGTGTAAACGCAGCGGCTAAAGACCAAGTCTCT50.1849796522382538No Hit
GCAAAGTTCTCAGGGATGTGTAAGCGCAGCGGCTAAAGACCAAGTCTCTG50.1849796522382538No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACAC40.14798372179060304No Hit
GCAGCTTAGCAGAGATGTGTAAGCGCAGCGGCTAAAGACCAAGTCTCTGC40.14798372179060304No Hit
AGCACTTGAATCAACAGGCTAAAGACCAAGTCTCTGCTACCGTATTAGTT40.14798372179060304No Hit
GCAAAAGTTCTCAGGGATGTGTAAGCGCACGGCTAAAGACCAAGTCTCTG40.14798372179060304No Hit
GCAAAAGTTCTCAGAGATGTGTAAGCGCACGGCTAAAGACCAAGTCTCTG40.14798372179060304No Hit
GCAAAAGTTCTCAGGGATGTGTAAGCGCAGCCTTCCCATGTACTCTGCGT40.14798372179060304No Hit
AGCAACAATGATCAGCAGGCTAAAGACCAAGTCTCTGCTACAGTATTCGT40.14798372179060304No Hit
GCAAAAGTTCTCAGGGATGTGTAAGCGCAACGGCTAAAGACCAAGTCTCT40.14798372179060304No Hit
AGCAACAATGATCAACAGGCTAAAGACCAAGTCTCTGCTACCGTATTCGT40.14798372179060304No Hit
GCAAAAGTTCTCAGGGATGTCCGAGATGTGTAAGCGCAGCGGCTAAAGAC30.11098779134295228No Hit
AATCCTGTTTAGGCCTTGCGGCCACTGATAGGAGGCTAAAGACCAAGTCT30.11098779134295228No Hit
GCAAAAGTTCTCAGAGATGTGTAAACGCAGCGGCTAAAGACCAAGTCTCT30.11098779134295228No Hit
AGCACTTGAATCAACAGGCTACTGACGACATGGTTCTACAGTCCAAAGCA30.11098779134295228No Hit
GCAAAAGTTCTCAGGGATGTCCTATCTTGAGGTAGGCTTCCCGCTTAGAT30.11098779134295228No Hit
AGCATTGAATCAACAGGCTAAAGACCAAGTCTCTGCTACCGTATTCGTTC30.11098779134295228No Hit
AGCACTTAATCAACAGGCTAAAGACCAAGTCTCTGCTACCGTATTCGTTC30.11098779134295228No Hit
AACTCCTGTTTAGGCCTCTCTCCTCATTCTAAACAACGGCTAAAGACCAA30.11098779134295228No Hit
AGCAACAATGATCAGCAGGCTAAAGACCAAGTCTCTGCTACCCTCTCTCC30.11098779134295228No Hit
AGCACTTGAATCAACACACTGACGACATGGTTCTACAGCCCAAAGCAACA30.11098779134295228No Hit
AACTCCTGTTTAGGCCTTGCACTGACGACATGGTTCTACAGTCCAAAGCA30.11098779134295228No Hit
AGCAACAATGATCAGCAGGCTAAAGACCAAGTCTCTGCTACCGTAATCGT30.11098779134295228No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)