FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008145409

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008145409
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2728
Total Bases395.5 kbp
Sequences flagged as poor quality0
Sequence length142-145
%GC64

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTGATTCAAGTGCTTTGGACTGTAGAACCATGTCGTCAGTGTAGATCT71826.319648093841643No Hit
TGCTGATCATTGTTGCTTTGGACTGTAGAACCATGTCGTCAGTGTAGATC58121.29765395894428No Hit
GCTGCGCTTACACATCCCTGAGAACTTTTGCTTGGACTGTAGAACCATGT42015.395894428152493No Hit
GCTGCGCTTACACATCTCTGAGAACTTTTGCTTGGACTGTAGAACCATGT29010.63049853372434No Hit
TCCTATCAGTGGCCGCAAGGCCTAAACAGGAGTTTTGGACTGTAGAACCA2338.541055718475073No Hit
TCCTATCAGTGGCCGCAAAGCCTAAACAGGAGTTTTGGACTGTAGAACCA572.089442815249267No Hit
TGTTATTCAAGTGCTTTGGACTGTAGAACCATGTCGTCAGTGTAGATCTC220.8064516129032258No Hit
GCTGCGCTTACACATCTCTTCTAAGCAGTGGTATCAACGCAGAGTACATG180.6598240469208211Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTGCGCTTACACATCCCTGAGAACTTTGCTTGGACTGTAGAACCATGTC80.2932551319648094No Hit
GCTGCGCTTACACATCTCTGACATCCCTGAGAACTTTTGCTTGGACTGTA70.2565982404692082No Hit
GCTGCGCTTACACATCCTGAGAACTTTTGCTTGGACTGTAGAACCATGTC60.21994134897360706No Hit
GCTGCGCTTACACATCTCTGCTAAGCAGTGGTATCAACGCAGAGTACATG50.18328445747800587Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTGTTTAGAATGAGGAGAGAGGGTAGCAGAGACTTGGTCTTTAGCCTGT50.18328445747800587No Hit
GCTGCGCTTACACATCTCTGAGAACTTTGCTTGGACTGTAGAACCATGTC50.18328445747800587No Hit
TGTTGATTCAAGTGCTTTTGGACTGTAGAACCATGTCGTCAGTGTAGATC50.18328445747800587No Hit
TGTGATCATTGTTGCTTTGGACTGTAGAACCATGTCGTCAGTGTAGATCT50.18328445747800587No Hit
TGTTGATTCAAGTGCTTTGGACTGTAGAACCATGCGTCAGTGTAGATCTC40.1466275659824047No Hit
TGCTGATCATTGTTGCTTTGGACTGTAGAACCATGTCGTCAGTGTGTTGA40.1466275659824047No Hit
TGTTGATCATTGTTGCTTTGGACTGTAGAACCATGTCGTCAGTGTAGATC40.1466275659824047No Hit
GCTGCGCTTACACATCCCTGAAACTTTTGCTTGGACTGTAGAACCATGTC40.1466275659824047No Hit
GCTGCGTTTACACATCCCTGAGAACTTTTGCTTGGACTGTAGAACCATGT40.1466275659824047No Hit
TGCTGATCATTGTTGCTTTGGACTGTAGAACCATGTCGTCAGTGTTGATT40.1466275659824047No Hit
GCTGTGCTTACACATCCCTGAGAACTTTTGCTTGGACTGTAGAACCATGT40.1466275659824047No Hit
TGTGATTCAAGTGCTTTGGACTGTAGAACCATGTCGTCAGTGTAGATCTC30.10997067448680353No Hit
TGCTGATATTGTTGCTTTGGACTGTAGAACCATGTCGTCAGTGTAGATCT30.10997067448680353No Hit
TGCTGTCATTGTTGCTTTGGACTGTAGAACCATGTCGTCAGTGTAGATCT30.10997067448680353No Hit
TCCTATCAGTGGCCTAAACAGGAGTTTTGGACTGTAGAACCATGTCGTCA30.10997067448680353No Hit
GCTGCGTTTACACATCTCTGAGAACTTTTGCTTGGACTGTAGAACCATGT30.10997067448680353No Hit
TCCTATCAGTGGCCGCAGGCCTAAACAGGAGTTTTGGACTGTAGAACCAT30.10997067448680353No Hit
TGCTGATCATTGTTGCTTTGGGCTGTAGAACCATGTCGTCAGTGTGTTGA30.10997067448680353No Hit
TCCTATCAGTGGCGCAAGGCCTAAACAGGAGTTTTGGACTGTAGAACCAT30.10997067448680353No Hit
TGTTGATCAAGTGCTTTGGACTGTAGAACCATGTCGTCAGTGTAGATCTC30.10997067448680353No Hit
TGCTGGATCATTGTTGCTTTGGACTGTAGAACCATGTCGTCAGTGTAGAT30.10997067448680353No Hit
TGTTGATTCAAGTGCTTTGGACTGTAGAACCATGTCGTCAGTGTGTTGAT30.10997067448680353No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)