FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008145427

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008145427
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22107
Total Bases3.2 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC873439.50784819288008No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT752334.029945266205274No Hit
TCCTATCAGTGGCCGCAAGGCCTAAACAGGAGTTCCATGTTGTAGAACCA6252.8271588184737864No Hit
GCTGCGCTTACACATCCCTGAGAACTTTTGCCCATGTTGTAGAACCATGT5692.573845388338535No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAAATCAAGTCAAATTT4602.0807888903967067No Hit
GCTGCGCTTACACATCTCTGAGAACTTTTGCCCATGTTGTAGAACCATGT4432.00389017053422No Hit
GTTGTTTAGAATGAGGAGAGAGGCCCTGGGCTTCACTTACTCTTCATTCA2841.2846609671144886No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAATCAAGTCAAATTTC2210.999683358212331No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAAAATCAAGTCAAATT1280.5790021260234315No Hit
TGCTGATCATTGTTGCTTTGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT780.35282942054552857No Hit
TCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT680.307594879449948No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAATCAAGTCAAATTTCT670.30307142534038994No Hit
TCCTATCAGTGGCCGCAAGGAGGGGAGAGGCCCTGGGCTTCACTTACTCT580.2623603383543674No Hit
TGCGGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC570.25783688424480933No Hit
TCCTATCAGTGGCCGCAAAGCCTAAACAGGAGTTCCATGTTGTAGAACCA530.2397430678065771No Hit
TCCTATCAGTGGCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT430.19450852671099653No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAGGTCTGGAGTATTTCCTC370.16736780205364815No Hit
TCCTATCATGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT340.153797439724974No Hit
TCCTACAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT310.14022707739629983No Hit
TCCTATCAGTGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT300.13570362328674174No Hit
TCCTATCGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT290.1311801691771837No Hit
TCCTTCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT290.1311801691771837No Hit
TCCTATAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT290.1311801691771837No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAAAAATCAAGTCAAAT270.12213326095806758No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAGAAGTCTGGAGTATTTCCTC260.11760980684850952No Hit
TGCTGATCATTGTTGCTTTGGGGGTGGGGAAAAGTCTGGAGTATTTCCTC250.11308635273895146No Hit
TCCTATCAGGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT240.1085628986293934No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGGGGCCCTGGGCTTCACTTACTCT240.1085628986293934No Hit
TGTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT230.10403944451983535No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTCCACTTACTCT230.10403944451983535No Hit
GCTGCGCTTACACATCTCTTCTAAGCAGTGGTATCAACGCAGAGTACATG230.10403944451983535Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)