FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008145461

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008145461
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20013
Total Bases2.9 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC898644.90081447059411No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT592329.595762754209765No Hit
GCTGCGCTTACACATCTCTTCCACTTCTTTTACTCTGGCATATTTGAGAC7723.8574926297906362No Hit
GCTGCGCTTACACATCTCTGAGAACTTTTGCCCATGTTGTAGAACCATGT4942.4683955428971167No Hit
TCCTATCAGTGGCCGCAAGGCCTAAACAGGAGTTCCATGTTGTAGAACCA4122.05866186978464No Hit
GCTGCGCTTACACATCCCTGAGAACTTTTGCCCATGTTGTAGAACCATGT3581.7888372557837404No Hit
GTTGTTTAGAATGAGGAGAGAGGCCCTGGGCTTCACTTACTCTTCATTCA1870.9343926447808925No Hit
CAACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACA1660.8294608504472093No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACCTACTCT720.3597661520011992No Hit
TGCTGATCATTGTTGCTTTGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT680.33977914355668815No Hit
TGCGGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC590.29480837455653824No Hit
TCCTATCAGTGGCCGCAAGGAGGGGAGAGGCCCTGGGCTTCACTTACTCT430.21486034077849397No Hit
TCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT430.21486034077849397No Hit
GCTGCGCTTACACATCTCTGACATCCCTGAGAACTTTTGCCCATGTTGTA430.21486034077849397No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAGGTCTGGAGTATTTCCTC420.2098635886673662No Hit
TCCTATAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT390.1948733323339829No Hit
TGCTGATCATTGTTGTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT370.18487982811172737No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAGAAGTCTGGAGTATTTCCTC360.1798830760005996No Hit
TCCTATCAGTGGCCGCAAAGCCTAAACAGGAGTTCCATGTTGTAGAACCA340.16988957177834407No Hit
TCCTATCAGTGGCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT330.1648928196672163No Hit
TCCTATCGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT280.13990905911157747No Hit
GCTGCGCTTACAATCTCTTCCACTTCTTTTACTCTGGCATATTTGAGACT270.1349123070004497No Hit
TCCTATCATGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT260.12991555488932194No Hit
GCTGCGCTTACACATCTCTTCTAAGCAGTGGTATCAACGCAGAGTACATG260.12991555488932194Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTACTTCCTC250.12491880277819417No Hit
TGTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT240.11992205066706642No Hit
TGCTGATCATGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT230.11492529855593864No Hit
TGCTGATATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT230.11492529855593864No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCCTCACTTACTCT220.10992854644481087No Hit
GCTGCGCTTACACATCTCGGACATCCCTGAGAACTTTTGCCCATGTTGTA220.10992854644481087No Hit
TCCTTCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT220.10992854644481087No Hit
TGCTGATCATTGTTGCTTTGGGGGTGGGGAAAAGTCTGGAGTATTTCCTC210.1049317943336831No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)