FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008145474

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008145474
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15998
Total Bases2.3 Mbp
Sequences flagged as poor quality0
Sequence length143-145
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA788749.29991248906113No Hit
AACTCCTGTTTAGGCCTTGCAGAATTTGGGACTCTGCCATATTCTTTCCG395724.73434179272409No Hit
GCAAAAGTTCTCAGGGATGTGTAAGCGCAGCTCACAGAGACCAAGTCTCT5183.2379047380922614No Hit
AACTCCTGTTTAGGCCTTGCGGCCACTGATAGGATCACAGAGACCAAGTC5073.1691461432679082No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACAC4792.9941242655331917No Hit
GCAAAAGTTCTCAGAGATGTGTAAGCGCAGCTCACAGAGACCAAGTCTCT3552.2190273784223025No Hit
GGTGTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG970.6063257907238405No Hit
GGTGTTTGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG930.5813226653331667No Hit
GTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG920.5750718839854981No Hit
AACTCCTGTTTAGGCTTTGCGGCCACTGATAGGATCACAGAGACCAAGTC750.46880860107513433No Hit
GGTGTTTGGGATGGATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG410.2562820352544068No Hit
AACTCCTGTTAGGCCTTGCAGAATTTGGGACTCTGCCATATTCTTTCCGT380.23752969121140144No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTAGCAGAAGCAGCA350.21877734716839606No Hit
AACTCCTGTTTAGGCCTTGCAGAATTTGGGACTCTACCATATTCTTTCCG340.2125265658207276No Hit
AACTCTGTTTAGGCCTTGCAGAATTTGGGACTCTGCCATATTCTTTCCGT340.2125265658207276No Hit
ACTCCTGTTTAGGCCTTGCAGAATTTGGGACTCTGCCATATTCTTTCCGT320.20002500312539068No Hit
GGTGTTTGGGATGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG280.17502187773471684No Hit
GGTTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG270.1687710963870484No Hit
GGTGTTTGGGTGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG270.1687710963870484No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGTA230.14376797099637453No Hit
GGGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG220.1375171896487061No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCGGCA210.1312664083010376No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAGGCAGCA210.1312664083010376No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGCTCGCAGAAGCAGCA200.12501562695336918No Hit
GCAAAAGTTCTCAGGGATGTCAGAGATGTGTAAGCGCAGCTCACAGAGAC190.11876484560570072No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCGGGTGGTTCGCAGAAGCAGCA190.11876484560570072No Hit
GGTGTTTGGGATGGAATGGTGATCCGCGCAGGTGGTTCGCAGAAGCAGCA190.11876484560570072No Hit
GGTGTTTGGGATGGAATGGTGACCCACGCAGGTGGTTCGCAGAAGCAGCA180.11251406425803225No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCACAGAAGCAGCA180.11251406425803225No Hit
AATCCTGTTTAGGCCTTGCAGAATTTGGGACTCTGCCATATTCTTTCCGT170.1062632829103638No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCGGAAGCAGCA170.1062632829103638No Hit
GGTGTTTGGGATGGAATGGTGGTCCACGCAGGTGGTTCGCAGAAGCAGCA160.10001250156269534No Hit
GGTGTTTGGGATGGAATGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG160.10001250156269534No Hit
GGTGTTTGGGAGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG160.10001250156269534No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGCGGTTCGCAGAAGCAGCA160.10001250156269534No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)