FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008145493

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008145493
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20248
Total Bases2.9 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT690434.097194784670094No Hit
TGCTGATCATTGTTGCTTTGGGGTTGAGGAAAAGTCTGGAGTATTTCCTC585028.891742394310548No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC323515.97688660608455No Hit
TCCTATCAGTGGCCGCAAGGCCTAAACAGGAGTTCCATGTTGTAGAACCA4532.2372580007902014No Hit
GCTGCGCTTACACATCTCTGAGAACTTTTGCCCATGTTGTAGAACCATGT3471.7137495061240615No Hit
GCTGCGCTTACACATCTCTTCCACTTCTTTTACTCTGGCATATTTGAGAC3311.6347293559857763No Hit
GTTGTTTAGAATGAGGAGAGAGGCCCTGGGCTTCACTTACTCTTCATTCA2691.3285262741999209No Hit
GCTGCGCTTACACATCCCTGAGAACTTTTGCCCATGTTGTAGAACCATGT2681.3235875148162781No Hit
TCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT750.3704069537732122No Hit
GCTGCGCTTACACATCTCTGACATCCCTGAGAACTTTTGCCCATGTTGTA440.2173054128802845No Hit
TCCTATAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT410.20248913472935598No Hit
TCCTATCAGTGGCCGCAAGGAGGGGAGAGGCCCTGGGCTTCACTTACTCT390.19261161596207033No Hit
TGCGGATCATTGTTGCTTTGGGGTTGAGGAAAAGTCTGGAGTATTTCCTC360.17779533781114185No Hit
TGCTGATCATTGTTGCTTTGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT340.16791781904385616No Hit
TGCTGATCATTGTTGCTTTGGGTTGAGGAAAAGTCTGGAGTATTTCCTCT320.1580403002765705No Hit
TCCTATCGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT310.15310154089292768No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGGGGCCCTGGGCTTCACTTACTCT300.14816278150928486No Hit
TCCTTCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT290.14322402212564203No Hit
TGCTGATCATTGTTGCTTTGGGGTTGAGGAAAGGTCTGGAGTATTTCCTC280.1382852627419992No Hit
GCTGCGCTTACACATCTCTTCTAAGCAGTGGTATCAACGCAGAGTACATG280.1382852627419992Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCTATCAGTGGCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT260.12840774397471355No Hit
TCCTATCAGTGGCCGCAAAGCCTAAACAGGAGTTCCATGTTGTAGAACCA250.12346898459107074No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCCTCACTTACTCT240.1185302252074279No Hit
TGCGGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC210.10371394705649942No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)