FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008145526

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008145526
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20524
Total Bases2.9 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACAC900043.851101149873315No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA526125.63340479438706No Hit
AACTCCTGTTTAGGCCTTGCAGAATTTGGGACTCTGCCATATTCTTTCCG19999.739816799844085No Hit
AACTCCTGTTTAGGCCTTGCGGCCACTGATAGGATCACAGAGACCAAGTC6082.962385499902553No Hit
GCAAAAGTTCTCAGAGATGTGTAAGCGCAGCTCACAGAGACCAAGTCTCT3961.929448450594426No Hit
GCAAAAGTTCTCAGGGATGTGTAAGCGCAGCTCACAGAGACCAAGTCTCT3571.7394270122783082No Hit
CGCCAGGCAGGAATCATTCAGGCCTTGCAGAATTTGGGACTCTGCCATAT1370.667511206392516No Hit
AACTCCTGTTTAGGCTTTGCGGCCACTGATAGGATCACAGAGACCAAGTC690.3361917754823621No Hit
GGTGTTTGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG630.30695770804911326No Hit
AGCACTTGATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC550.26797895147144807No Hit
GGTGTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG540.2631066068992399No Hit
GTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG530.2582342623270318No Hit
AGACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC470.22900019489378287No Hit
AGCACTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC450.21925550574936659No Hit
AGCACTTGAATAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC400.19489378288832587No Hit
AGCACTTGAAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACA370.18027674917170142No Hit
AGCACTTGAATCAACAGGCTCCAGAGACTGAGCCATTTTCAAACTCACAC370.18027674917170142No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACCCACAC330.16078737088286885No Hit
AGCATTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC320.15591502631066068No Hit
GCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC290.14129799259403625No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTATCAAACTCACAC270.13155330344961996No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGGGCCATTTTCAAACTCACAC270.13155330344961996No Hit
GCAAAAGTTCTCAGGGATGTCAGAGATGTGTAAGCGCAGCTCACAGAGAC250.12180861430520368No Hit
GGTGTTTGGGATGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG250.12180861430520368No Hit
AGCACTTGAATCACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC240.11693626973299552No Hit
AGCACTTGAATCAACAGGCTTCAGAGGCTGAGCCATTTTCAAACTCACAC240.11693626973299552No Hit
GGTGTTTGGGTGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG220.10719158058857922No Hit
AGCACTTGAACAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC220.10719158058857922No Hit
AACTCCTGTTAGGCCTTGCAGAATTTGGGACTCTGCCATATTCTTTCCGT210.10231923601637108No Hit
AGCACTTAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC210.10231923601637108No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)