FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008145529

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008145529
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21778
Total Bases3.1 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC921742.322527321149785No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT469421.553861695288823No Hit
GCTGCGCTTACACATCTCTTCCACTTCTTTTACTGGCATATTTGAGACTT10935.01882633850675No Hit
GCTGCGCTTACACATCTCTGAGAACTTTTGCCCATGTTGTAGAACCATGT7283.342823032418037No Hit
TCCTATCAGTGGCCGCAAGGCCTAAACAGGAGTTCCATGTTGTAGAACCA6042.7734410873358435No Hit
GCTGCGCTTACACATCCCTGAGAACTTTTGCCCATGTTGTAGAACCATGT5452.502525484433832No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAAATCAAGTCAAATTT5232.40150610708054No Hit
GCTGCGCTTACACATCTCTTCCACTTCTTTTACTCTGGCATATTTGAGAC3341.5336578198181652No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAATCAAGTCAAATTTC3091.4188630728257874No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAAAATCAAGTCAAATT1990.913766186059326No Hit
GTTGTTTAGAATGAGGAGAGAGGCCCTGGGCTTCACTTACTCTTCATTCA1250.5739737349618882No Hit
TGCTGATCATTGTTGCTTTGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT1010.4637707778492056No Hit
GCTGCGCTTACACATCTCTGACATCCCTGAGAACTTTTGCCCATGTTGTA950.43622003857103503No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAATCAAGTCAAATTTCT860.394893929653779No Hit
TCCTATCAGTGGCCGCAAAGCCTAAACAGGAGTTCCATGTTGTAGAACCA590.2709156029020112No Hit
TCCTATCAGTGGCCGCAAGGAGGGGAGAGGCCCTGGGCTTCACTTACTCT450.20663054458627972No Hit
GCTGCGCTTACACATCTCGGACATCCCTGAGAACTTTTGCCCATGTTGTA440.20203875470658464No Hit
TGCGGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC440.20203875470658464No Hit
GCTGCGCTTACACATCTCTTCCACTTCTTTTATTGGCATATTTGAGACTT430.1974469648268895No Hit
GCTGCGCTTACAATCTCTTCCACTTCTTTTACTGGCATATTTGAGACTTT370.1698962255487189No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAAAAATCAAGTCAAAT320.14693727615024335No Hit
GCTGCGCTTACACATCTCTTCTAAGCAGTGGTATCAACGCAGAGTACATG310.14234548627054824Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAGGTCTGGAGTATTTCCTC300.13775369639085316No Hit
TGCTGATCATTGTTGTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT290.13316190651115806No Hit
TGCTGATCATTGTTGCTTTGGGGGTGGGGAAAAGTCTGGAGTATTTCCTC290.13316190651115806No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTCCCTC290.13316190651115806No Hit
TCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT280.12857011663146295No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGGGTATTTCCTC270.12397832675176783No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAGAAGTCTGGAGTATTTCCTC260.11938653687207272No Hit
TCCTATCAGTGGCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT260.11938653687207272No Hit
TGCTGATCTTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT260.11938653687207272No Hit
TCCTATCAGTGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT250.11479474699237761No Hit
TGCTATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT220.10101937735329232No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)