FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008145582

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008145582
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1524
Total Bases220.8 kbp
Sequences flagged as poor quality0
Sequence length141-145
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAAAAGTTCTCAGAGATGTGTAAGCGCAGCTCACAGAGACCAAGTCTCT41927.49343832020997No Hit
GCAAAAGTTCTCAGGGATGTGTAAGCGCAGCTCACAGAGACCAAGTCTCT37024.278215223097114No Hit
CGCCAGGCAGGAATCATTCAGGCCTTGCAGAATTTGGGACTCTGCCATAT1308.530183727034121No Hit
GCAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGC795.183727034120735No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACAC352.2965879265091864No Hit
GCAAAAGTTCTCAGGGATGTCAGAGATGTGTAAGCGCAGCTCACAGAGAC332.1653543307086616No Hit
GCAAAAGTTCTCAGGGATGTGTAAGCGCAGCCTTCCCATGTACTCTGCGT271.7716535433070866No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA191.246719160104987No Hit
GCAAAGTTCTCAGGGATGTGTAAGCGCAGCTCACAGAGACCAAGTCTCTG130.8530183727034121No Hit
GCAAAAGTTCTCAGGGATGTTAGAGATGTGTAAGCGCAGCTCACAGAGAC130.8530183727034121No Hit
GCAAAGTTCTCAGAGATGTGTAAGCGCAGCTCACAGAGACCAAGTCTCTG110.7217847769028871No Hit
CGCCAGGCAGGAATCATTCACACTGACGACATGGTTCTACAACATGGGCA100.6561679790026247No Hit
GCAAAAGTTCTCAGGGATGTCCGAGATGTGTAAGCGCAGCTCACAGAGAC90.5905511811023622No Hit
GCAAAAGTTCTCAGAGATGTGTAAGCGCAGCTCACAGAGACAAAGTCTCT70.45931758530183725No Hit
GCAAAAGTTCTCAGGGATGTCGATGAGGGGATCCCATGTACTCTGCGTTG70.45931758530183725No Hit
GCAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTG70.45931758530183725No Hit
GAAAAGTTCTCAGGGATGTGTAAGCGCAGCTCACAGAGACCAAGTCTCTG60.39370078740157477No Hit
AACTCCTGTTTAGGCCTTGCAGAATTTGGGACTCTGCCATATTCTTTCCG60.39370078740157477No Hit
GCAAAAGTTCTCAGGGATGTCACACTGACGACATGGTTCTACAACATGGG50.32808398950131235No Hit
GCAAAAGTTCTCAGGGATGTGTAAGCGCAGCTCACAGAGACAAAGTCTCT50.32808398950131235No Hit
CAAAAGTTCTCAGGGATGTGTAAGCGCAGCTCACAGAGACCAAGTCTCTG50.32808398950131235No Hit
GCAAAAGTCTCAGGGATGTGTAAGCGCAGCTCACAGAGACCAAGTCTCTG40.26246719160104987No Hit
GAAAAGTTCTCAGGGATGTCAGAGATGTGTAAGCGCAGCTCACAGAGACC40.26246719160104987No Hit
GAAAAGTTCTCAGAGATGTGTAAGCGCAGCTCACAGAGACCAAGTCTCTG40.26246719160104987No Hit
GCAAAAGTTCTCAGGATGTGTAAGCGCAGCTCACAGAGACCAAGTCTCTG40.26246719160104987No Hit
GCCAGGCAGGAATCATTCAGGCCTTGCAGAATTTGGGACTCTGCCATATT30.19685039370078738No Hit
GCAAAAGTTTCAGGGATGTGTAAGCGCAGCTCACAGAGACCAAGTCTCTG30.19685039370078738No Hit
GCAAAAGTTCTCAGAGATGTGTAAGCGCAGCTCACAGAGACCAAGTCTCA30.19685039370078738No Hit
GCAAAAGTCTCAGAGATGTGTAAGCGCAGCTCACAGAGACCAAGTCTCTG30.19685039370078738No Hit
GCAAAAGTTCTCAGGGATGTTGATGAGGGGATCCCATGTACTCTGCGTTG30.19685039370078738No Hit
GCAAAAGTTCTCAGGGATGTCCCACTGACGACATGGTTCTACAACATGGG30.19685039370078738No Hit
CAAAAGTTCTCAGAGATGTGTAAGCGCAGCTCACAGAGACCAAGTCTCTG30.19685039370078738No Hit
GCAAAAGTTCTCAGAGATGTGTAAGCGCAAGCTCACAGAGACCAAGTCTC30.19685039370078738No Hit
GCAAAAGTTCTCAGAGATGTGTAACGCAGCTCACAGAGACCAAGTCTCTG30.19685039370078738No Hit
GCAAAAGTTCTCAGGGATGTCGAGATGTGTAAGCGCAGCTCACAGAGACC30.19685039370078738No Hit
GCAAAAGTTCTCAGAGATGTGTAAGCGCAGCTCACAGAGACCAACTCTCT30.19685039370078738No Hit
CGCAGGCAGGAATCATTCAGGCCTTGCAGAATTTGGGACTCTGCCATATT30.19685039370078738No Hit
GCAAAAGTTCTCAGGGATGTGTAAGCGCAGCCTTGACGGCGCCGGCGACA30.19685039370078738No Hit
GCAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGCA30.19685039370078738No Hit
GCAAAAGTTCTCAGGGATGTCTCGTGGGTCAGAGATGTGTAAGCGCAGCT20.13123359580052493No Hit
GCAAAGTTCTCAGGGATGTGTAAGCGCAGCCTTCCCATGTACTCTGCGTT20.13123359580052493No Hit
GCAAAATTCTCAGGGATGTGTAAGCGCAGCTCACAGAGACCAAGTCTCTG20.13123359580052493No Hit
GCAAAAGTTCTCAGGGATGTGTAAGCGAAGAGATGTGTAAGCGCAGCTCA20.13123359580052493No Hit
GCAAAAGTTCTCAGGGATGTGTAAGAGCAGCTCACAGAGACCAAGTCTCT20.13123359580052493No Hit
GCAAAAGTTCTCAGGGATGTGTAAGCGCAGCCTTAGACGGCGCCGGCGAC20.13123359580052493No Hit
GCAAAAGTTCTCAGGGATGTCCTCTGCGTTGATACCACTGCTTAGAAGAG20.13123359580052493Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCAAAAGTTCTCAGTGATGTGTAAGCGCAGCTCACAGAGACCAAGTCTCT20.13123359580052493No Hit
GCAAAGTTCTCAGGGATGTCGCGGTGAGTTCAGGCTTTTTCATGGTTTAG20.13123359580052493No Hit
GCAAAAGTTCTCAGGGATGTGTAAGCGCACTCACAGAGACCAAGTCTCTG20.13123359580052493No Hit
GCAAAAGTTCTAGGGATGTGTAAGCGCAGCTCACAGAGACCAAGTCTCTG20.13123359580052493No Hit
GCAAAAGTTCTCAGGGATGTGTAAACGCAGCTCACAGAGACCAAGTCTCT20.13123359580052493No Hit
CGCCAGGCAGGATCATTCAGGCCTTGCAGAATTTGGGACTCTGCCATATT20.13123359580052493No Hit
GCAAAAGTTCTAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGCA20.13123359580052493No Hit
CGCCAGGCAGGAATCATTCACACTGACGACATGGTTCTACAACATGGCGC20.13123359580052493No Hit
AGCACTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC20.13123359580052493No Hit
CGCCAGGCAGGAATCACACTGACGACATGGTTCTACAACATGGGCAAAAG20.13123359580052493No Hit
GCAAAAGTTCTCAGGGATGTCGATGAGGTCATCCCCAAGCAGCTTATCGA20.13123359580052493No Hit
GCAAAGTTCTCAGGGATGTCCGAGATGTGTAAGCGCAGCTCACAGAGACC20.13123359580052493No Hit
ACAAAAGTTCTCAGAGATGTGTAAGCGCAGCTCACAGAGACCAAGTCTCT20.13123359580052493No Hit
GCAAAAGTGCTCAGGGATGTGTAAGCGCAGCTCACAGAGACCAAGTCTCT20.13123359580052493No Hit
GCAAAAGTTCTCAGGGATGTAAGAGATGTGTAAGCGCAGCTCACAGAGAC20.13123359580052493No Hit
GCAAAAGTTCTAGAGATGTGTAAGCGCAGCTCACAGAGACCAAGTCTCTG20.13123359580052493No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)