FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008145590

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008145590
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20348
Total Bases2.9 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACAC886443.56202083742874No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA388819.10752899547867No Hit
AACTCCTGTTTAGGCCTTGCAGAATTTGGGACTCTGCCATATTCTTTCCG282613.888342834676626No Hit
AACTCCTGTTTAGGCCTTGCGGCCACTGATAGGATCACAGAGACCAAGTC6673.277963436209947No Hit
CGCCAGGCAGGAATCATTCAGGCCTTGCAGAATTTGGGACTCTGCCATAT5042.476901906821309No Hit
GCAAAAGTTCTCAGAGATGTGTAAGCGCAGCTCACAGAGACCAAGTCTCT4712.3147238057794377No Hit
GCAAAAGTTCTCAGGGATGTGTAAGCGCAGCTCACAGAGACCAAGTCTCT4392.157460192647926No Hit
AACTCCTGTTTAGGCTTTGCGGCCACTGATAGGATCACAGAGACCAAGTC740.3636721053666208No Hit
AGCACTTGATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC480.23589541969726754No Hit
GGTGTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG450.2211519559661883No Hit
GTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG450.2211519559661883No Hit
GCAAAAGTTCTCAGGGATGTGTAAGCGCAGCCTTCCCATGTACTCTGCGT420.20640849223510913No Hit
AGACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC410.20149400432474934No Hit
AGCACTTGAAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACA400.19657951641438962No Hit
AGCACTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC390.1916650285040299No Hit
GCAAAAGTTCTCAGGGATGTCAGAGATGTGTAAGCGCAGCTCACAGAGAC380.18675054059367013No Hit
AGCACTTGAATCAACAGGCTCCAGAGACTGAGCCATTTTCAAACTCACAC380.18675054059367013No Hit
AGCACTTGAATAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC350.17200707686259092No Hit
AACTCCTGTTAGGCCTTGCAGAATTTGGGACTCTGCCATATTCTTTCCGT330.16217810104187144No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACCCACAC290.14252014940043248No Hit
AACTCTGTTTAGGCCTTGCAGAATTTGGGACTCTGCCATATTCTTTCCGT270.132691173579713No Hit
GGTGTTTGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG270.132691173579713No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCGCAC260.12777668566935327No Hit
AGCACTTGAATCACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC260.12777668566935327No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGGGCCATTTTCAAACTCACAC240.11794770984863377No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTCCAAACTCACAC210.10320424611755456No Hit
GGTGTTTGGGATGGATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG210.10320424611755456No Hit
AGCACTTGAATCAACAGGCTTCGGAGACTGAGCCATTTTCAAACTCACAC210.10320424611755456No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)