FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008145618

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008145618
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20819
Total Bases3 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACAC866141.601421778183386No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA486723.377683846486384No Hit
AACTCCTGTTTAGGCCTTGCAGAATTTGGGACTCTGCCATATTCTTTCCG219310.533647149238675No Hit
AACTCCTGTTTAGGCCTTGCGGCCACTGATAGGATCACAGAGACCAAGTC7203.4583793650031227No Hit
GCAAAAGTTCTCAGAGATGTGTAAGCGCAGCTCACAGAGACCAAGTCTCT5052.425668860175801No Hit
GCAAAAGTTCTCAGGGATGTGTAAGCGCAGCTCACAGAGACCAAGTCTCT4742.2767664152937224No Hit
CGCCAGGCAGGAATCATTCAGGCCTTGCAGAATTTGGGACTCTGCCATAT4402.113454056390797No Hit
AACTCCTGTTTAGGCTTTGCGGCCACTGATAGGATCACAGAGACCAAGTC860.4130842019309285No Hit
GCAAAAGTTCTCAGGGATGTGTAAGCGCAGCCTTCCCATGTACTCTGCGT690.3314280224794659No Hit
GGTGTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG610.29300158509054225No Hit
GTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG610.29300158509054225No Hit
AGACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC530.2545751477016187No Hit
GGTGTTTGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG470.225755319659926No Hit
GCAAAAGTTCTCAGGGATGTCAGAGATGTGTAAGCGCAGCTCACAGAGAC430.20654210096546424No Hit
AGCACTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC420.2017387962918488No Hit
GCAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGC410.19693549161823334No Hit
AGCACTTGATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC390.18732888227100244No Hit
AGCACTTGAATAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC370.17772227292377155No Hit
AGCACTTGAATCAACAGGCTCCAGAGACTGAGCCATTTTCAAACTCACAC330.15850905422930978No Hit
AGCACTTGAATCACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC310.14890244488207888No Hit
AGCACTTGAATCAACAGGCTTCGGAGACTGAGCCATTTTCAAACTCACAC270.12968922618761708No Hit
GGTGTTTGGGATGGATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG260.12488592151400164No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACCCACAC260.12488592151400164No Hit
AACTCCTGTTAGGCCTTGCAGAATTTGGGACTCTGCCATATTCTTTCCGT240.11527931216677074No Hit
AGCACTTGAAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACA240.11527931216677074No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGTCATTTTCAAACTCACAC240.11527931216677074No Hit
GCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC230.1104760074931553No Hit
AGCACTTAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC230.1104760074931553No Hit
GGTGTTTGGGATGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG220.10567270281953985No Hit
AGCACTTGAATCAACAGGTTTCAGAGACTGAGCCATTTTCAAACTCACAC220.10567270281953985No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)