FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008145625

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008145625
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18356
Total Bases2.6 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC887048.32207452604053No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT550429.984746132054912No Hit
TCCTATCAGTGGCCGCAAGGCCTAAACAGGAGTTCCATGTTGTAGAACCA4432.4133798213118327No Hit
GCTGCGCTTACACATCTCTGAGAACTTTTGCCCATGTTGTAGAACCATGT4352.3697973414687294No Hit
GCTGCGCTTACACATCCCTGAGAACTTTTGCCCATGTTGTAGAACCATGT3992.1736761821747654No Hit
GTTGTTTAGAATGAGGAGAGAGGCCCTGGGCTTCACTTACTCTTCATTCA1540.8389627369797342No Hit
TGCTGATCATTGTTGCTTTGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT770.4194813684898671No Hit
TGCGGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC720.3922423185879277No Hit
GCTGCGCTTACACATCTCTGACATCCCTGAGAACTTTTGCCCATGTTGTA490.2669426890390063No Hit
TCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT470.25604706907823055No Hit
TCCTATCAGTGGCCGCAAAGCCTAAACAGGAGTTCCATGTTGTAGAACCA410.22336020919590324No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAGGTCTGGAGTATTTCCTC390.2124645892351275No Hit
GCTGCGCTTACACATCTCTTCTAAGCAGTGGTATCAACGCAGAGTACATG390.2124645892351275Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT330.17977772935280018No Hit
TCCTTCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT330.17977772935280018No Hit
TCCTATCAGTGGCCGCAAGGAGGGGAGAGGCCCTGGGCTTCACTTACTCT320.1743299193724123No Hit
TCCTATAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT300.1634342994116365No Hit
GCTGCGCTTACACATCTCTGAGAACTTTTGCCTACATCCCTGAGAACTTT290.15798648943124863No Hit
TGCTGATCATTGTTGCTTTGGGGGTGGGGAAAAGTCTGGAGTATTTCCTC260.141643059490085No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAGAAGTCTGGAGTATTTCCTC260.141643059490085No Hit
TGCTGATATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT260.141643059490085No Hit
TCCTATCAGGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT250.1361952495096971No Hit
TCCTATCAGTGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT230.12529962954892132No Hit
TCCTATCGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT230.12529962954892132No Hit
TCCTATCAGTGGCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT230.12529962954892132No Hit
TGCTGATCATGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT230.12529962954892132No Hit
TGCTGATCATTGTTGTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT220.11985181956853344No Hit
TGCTGATCATTGTTGCTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT220.11985181956853344No Hit
TGCTGATCTTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT220.11985181956853344No Hit
TCCTATCATGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT210.11440400958814556No Hit
TCCTATCAGTGGCCGCAAAGAGGGGGGAGGCCCTGGGCTTCACTTACTCT210.11440400958814556No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATCTCCTC210.11440400958814556No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGGGTATTTCCTC200.10895619960775767No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAGAGTCTGGAGTATTTCCTC190.1035083896273698No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGGGGCCCTGGGCTTCACTTACTCT190.1035083896273698No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)