FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008145671

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008145671
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22461
Total Bases3.2 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC792435.27892791950492No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT701431.227460932282625No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAAATCAAGTCAAATTT8603.828858911001291No Hit
TCCTATCAGTGGCCGCAAGGCCTAAACAGGAGTTCCATGTTGTAGAACCA5862.608966653310182No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAATCAAGTCAAATTTC5522.4575931614798985No Hit
GCTGCGCTTACACATCCCTGAGAACTTTTGCCCATGTTGTAGAACCATGT4822.145941854770491No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCA4391.9544989092204264No Hit
GCTGCGCTTACACATCTCTGAGAACTTTTGCCCATGTTGTAGAACCATGT4231.883264324829705No Hit
GTTGTTTAGAATGAGGAGAGAGGCCCTGGGCTTCACTTACTCTTCATTCA2691.1976314500690084No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAAAATCAAGTCAAATT2641.1753706424469081No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAATCAAGTCAAATTTCT1810.8058412359200393No Hit
TGCTGATCATTGTTGCTTTGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT780.3472685989047683No Hit
TCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT660.29384266061172704No Hit
TGCGGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC600.2671296914652063No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAAAAATCAAGTCAAAT490.21815591469658518No Hit
TCCTATCAGTGGCCGCAAAGCCTAAACAGGAGTTCCATGTTGTAGAACCA480.21370375317216506No Hit
TCCTATCAGTGGCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT450.20034726859890478No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAGGTCTGGAGTATTTCCTC440.19589510707448465No Hit
TCCTATCAGTGGCCGCAAGGAGGGGAGAGGCCCTGGGCTTCACTTACTCT420.18699078402564445No Hit
TCCTATAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT410.18253862250122435No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAATCAAGTCAAATTTCTT370.16472997640354392No Hit
TCCTATCAGTGGCCGCAAAGAGGGGGGAGGCCCTGGGCTTCACTTACTCT310.1380170072570233No Hit
TCCTATCATGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT300.13356484573260316No Hit
TCCTATCGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT280.12466052268376296No Hit
GCTGCGCTTACACATCTCTTCTAAGCAGTGGTATCAACGCAGAGTACATG280.12466052268376296Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCTACAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT260.11575619963492276No Hit
TCCTATCAGTGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT260.11575619963492276No Hit
TCCTATCAGGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT250.11130403811050264No Hit
TGCTGATCATTGTTGCTTTGGGGGTGGGGAAAAGTCTGGAGTATTTCCTC250.11130403811050264No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAGAAGTCTGGAGTATTTCCTC230.10239971506166244No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTCCCTC230.10239971506166244No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)